中国药房2013，Vol.24Issue(21)：1950-1952,3.DOI:10.6039/j.issn.1001-0408.2013.21.11
根皮苷和3-羟基根皮苷在Caco-2细胞单层模型中的转运与吸收研究
Study on Absorption and Transportation of Phloridzin and 3-Hydroxy-phloridzin in Caco-2 Cell Monolayer Model
罗明琍 1黄松 2吴新荣2
作者信息
- 1. 广州军区广州总医院,广州 510010
- 2. 广州中医药大学,广州 510006
- 折叠
摘要
Abstract
OBJECTIVE:To study absorption and transportation of phloridzin and 3-hydroxy-phloridzin in cloning of human colon adenocarcinoma cells (Caco-2 cell) monolayer model.METHODS:HPLC method was adopted.Using apparent permeation coefficient Papp as index,two-way absorption and transportation (AP→BL and BL→AP) of phloridzin and 3-hydroxy-phloridzin (50,100,200 μmol/L) in Caco-2 cell monolayer model were investigated.Simultaneous transportation of drug combination was compared with that of single drug; the interaction of the two drugs was investigated.RESULTS:With the increase of concentration,Papp of phloridzin (AP→BL and BL→AP) increased to some extent; Papp of 3-hydroxy-phloridzin (AP→BL) decreased to some extent,while that of 3-hydroxy-phloridzin (BL→AP) increased slightly.Compared with single transportation,transportation amount and Papp of phloridzin combined with 3-hydroxy-phloridzin decreased significantly (P＜0.05).CONCLUSIONS:Phloridzin and 3-hydroxy-phloridzin have good permeability coefficient on Caco-2 cell monolayer model.When transport together,competitive inhibition is found between two drugs.关键词
根皮苷/3-羟基根皮苷/Caco-2细胞单层模型/吸收/转运Key words
Phloridzin/ 3-Hydroxy-phloridzin/ Caco-2 cell monolayer model/ Absorption/ Transportation分类
医药卫生引用本文复制引用
罗明琍,黄松,吴新荣..根皮苷和3-羟基根皮苷在Caco-2细胞单层模型中的转运与吸收研究[J].中国药房,2013,24(21):1950-1952,3.
