中国药房2013，Vol.24Issue(23)：2138-2140,3.DOI:10.6039/j.issn.1001-0408.2013.23.10
原花青素对心肌缺血再灌注模型大鼠心肌细胞凋亡的影响
Effects of Procyanidin on Cardiocyte Apoptosis of Rats after Myocardial Ischemia Reperfusion
刘丹 1刘义1
作者信息
- 1. 辽宁医学院药理学教研室,辽宁锦州121001
- 折叠
摘要
Abstract
OBJECTIVE:To study the effects of procyanidin (PC) on cardiocyte apoptosis of myocardial ischemia-reperfusion injury in rats.METHODS:SD rats were randomly assigned into 4 groups:control group (constant volume of normal saline),model group (constant volume of normal saline) and PC high-dose and low-dose groups (100,50 mg/kg) via i.g.administration,once a day,for consecutive 2 weeks.After last administration,myocardial ischemia-reperfusion model was induced by ligating LADCA for 30 min followed by 120 min of perfusion.The activity of CK-MB was detected after experiment.The content of ROS was determined by ROS fluorescent probe dihydroethidium; the expressions of p53 and Caspase-9 were determined by Western blotting;myocardial apoptosis was measured by the method of TUNEL.RESULTS:Compared with control group,the contents of ROS,the expressions of p53 and Caspase-9 increased significantly in model group,and the cardiocyte apoptosis index increased significantly (P＜0.01) ; compared with model group,the content of ROS,the expressions of p53 and Caspase-9 decreased significantly,and the cardiocyte apoptosis index decreased significantly(P＜0.01).CONCLUSIONS:Procyanidin can reduce cardiocyte apoptosis after ischemia-reperfusion.This beneficial effect is partially dependant on ROS-p53-Caspase-9 signal transduction pathway.关键词
缺血再灌注/原花青素/信号转导/细胞凋亡Key words
Ischemia reperfusion/ Procyanidin/ Signal transduction/ Cardiocyte apoptosis分类
医药卫生引用本文复制引用
刘丹,刘义..原花青素对心肌缺血再灌注模型大鼠心肌细胞凋亡的影响[J].中国药房,2013,24(23):2138-2140,3.
