中国药房2013，Vol.24Issue(23)：2152-2154,3.DOI:10.6039/j.issn.1001-0408.2013.23.14
UPLC法同时测定黄连与代综方中4种生物碱的含量
Simultaneous Determination of 4 Kinds of Alkaloids from Coptidis Rhizoma and Daizongfang by UPLC
摘要
Abstract
OBJECTIVE:To establish the method for simultaneous determination ofjatrorrhizine,coptisine,palmatine and berberine,4 kinds of alkaloid in Coptidis Rhizoma and Daizongfang.METHODS:UPLC method was adopted.The determination was performed on ACQUITY UPLC BEH C18 (100 mm×2.1 mm,1.7 μm) column with mobile phase consisted of acetonitrile-water (2mmol ammonium acetate and 0.05％ formic acid,30∶70,V/V,pH=3.20) at the flow rate of 0.3 ml/min.The column temperature was 30 ℃,and detection wavelength was 345 nm.RESULTS:The liner ranges of jatrorrhizine,coptisine,palmatine and berberine hydrochloride were 21.76-1 088.05 ng/ml,20.88-1 044.00 ng/ml,17.68-884.05 ng/ml and 18.32-916.10 ng/ml (all r=0.999 9),respectively.RSDs of precision,stability and reproducibility test were all lower than 5％.The average recoveries of jatrorrhizine,coptisine,palmatine and berberine hydrochloride in Coptidis Rhizoma were 101.56％ (RSD=2.16％),100.21％ (RSD=3.32％),97.60％ (RSD=1.96％) and 98.81％ (RSD=1.48％),respectively(n=6).The average recoveries of 4 alkaloids in Daizongfang were 98.87％ (RSD=2.41％),99.12％ (RSD=2.68％),98.98％ (RSD=2.23％),98.55％ (RSD=2.82％),respectively(n=6).The lowest detectable and quantitative concentration of 4 alkaloids were 4.50 and 14.99 ng/ml,5.10 and 17.00 ng/ml,4.81 and 16.04ng/ml,4.64 and 15.47 ng/ml,respectively.CONCLUSIONS:The method is simple,accurate and reproducible.It can be used for the quality control of Coptidis Rhizoma and its component preparation.关键词
超高效液相色谱法/黄连/代综方/药根碱/黄连碱/巴马汀/小檗碱/含量测定Key words
UPLC/ Coptidis Rhizome/ Daizongfang/ Jatrorrhizine/ Coptisine/ Palmatine/ Berberine/ Content determination分类
医药卫生引用本文复制引用
陈健龙,张玉玲,肖胜利,崔翰明,贡济宇..UPLC法同时测定黄连与代综方中4种生物碱的含量[J].中国药房,2013,24(23):2152-2154,3.基金项目
国家科技重大新药创制专项资助课题(No.2011ZX09102-011-08) （No.2011ZX09102-011-08）
