中国药房2013，Vol.24Issue(23)：2172-2174,3.DOI:10.6039/j.issn.1001-0408.2013.23.21
白术及其麸炒品的HPLC指纹图谱比较研究
Comparison of HPLC Fingerprint between Atractylodes macrocephala and Stir-baked Atractylodes macrocephala with Bran
杨彦华 1沈晓庆 1赵文龙 1贾天柱1
作者信息
- 1. 辽宁中医药大学药学院,辽宁大连 116600
- 折叠
摘要
Abstract
OBJECTIVE:To develop fingerprint ofAtractylodes macrocephala and stir-baked Atractylodes macrocephala with bran,and to compare the difference of fingerprint between them.METHODS:HPLC method was adopted.The determination was performed on Diamonsil C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitril-water (gradient elution) at the flow rate of 0.8 ml/min.The detection wavelength was set at 242 nm,and the column temperature was 28 ℃.The analytic time was 100 min.RESULTS:Under the above chromatographic conditions,the mutual mode of HPLC fingerprints was set up for Atractylodes macrocephala; stir-baked Atractylodes macrocephala with bran had a similar fingerprint chromatogram to crude drug (similarity＞0.9),but there was a little difference in their concent; among 23 common peaks,No.6,10 and 3 peaks were identified as atractylenolide Ⅰ,Ⅱ,Ⅲ.CONCLUSIONS:Established fingerprint is in good precision,reproducibility and stability,which can be used as evidence for quality evalution ofAtractylodes macrocephala and processed product.关键词
白术/麸炒白术/高效液相色谱法/指纹图谱Key words
Atractylodes macrocephala/ Stir-baked Atractylodes macrocephala with bran/ HPLC/ Fingerprint分类
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杨彦华,沈晓庆,赵文龙,贾天柱..白术及其麸炒品的HPLC指纹图谱比较研究[J].中国药房,2013,24(23):2172-2174,3.
