中国药房2013，Vol.24Issue(25)：2360-2362,3.DOI:10.6039/j.issn.1001-0408.2013.25.20
离子色谱法测定反义寡核苷酸抗流感新药中铵残留量
Content Determination of Residual Ammonia in Antisense Oligonucleotide Antiviral Drugs by Ion Chromatography
摘要
Abstract
OBJECTIVE:To establish the method for the determination of residual ammonia in antisense oligonucleotide antiviral drugs (Flutide).METHODS:The sample was pre-treated by anion-exchange resin and removed interference from nucleic acid (monitored with UV spectrophotometry).The content of ammonia was determined by cation exchange chromatography.IonPac CS12 column was used with elutriant consisted of 12 mmol/L loprazolam at the flow rate of 1 ml/min.The current of suppressed conductivity detector was 59 mA.RESULTS:The linear range of ammonia ion were 0.6-30 μg/mL (r=0.999 5) with average recovery rate of 99.44％ (RSD=1.3％,n=6).The detection limit was 0.12 ng.CONCLUSIONS:The method is accurate and suitable for the residual ammonia control in macromolecule water-solubile antisense oligonucletide.关键词
反义寡核苷酸/离子色谱法/铵残留量/Flutide/测定Key words
Antisense oligonucleotide/ Ion chromatography/ Residual ammonia content/ Flutide/ Dertermination分类
医药卫生引用本文复制引用
仲婕,李保山,朱德领,张金钟,金艳,施明珠,殷爱军..离子色谱法测定反义寡核苷酸抗流感新药中铵残留量[J].中国药房,2013,24(25):2360-2362,3.基金项目
"十二五"国家重大新药创制-科技重大专项课题资助(No.2011ZX09102-009-09) （No.2011ZX09102-009-09）
