中国药房2013，Vol.24Issue(25)：2372-2374,3.DOI:10.6039/j.issn.1001-0408.2013.25.25
HPLC法同时测定复方坎地沙坦酯氢氯噻嗪片中的主成分含量
Simultaneous Determination of Main Components in Compound Hydrochlorothiazide/Candesartan Cilexetil Tablets by HPLC
谢鹏 1王敏1
作者信息
- 1. 唐山市协和医院药剂科,河北唐山063004
- 折叠
摘要
Abstract
OBJECTIVE:To estbalish the method for simultaneous determination of main components in Compound hydrochlorothiazide/candesartan cilexetil tablets.METHODS:HPLC method was adopted.The determination was performed on Venusil XBP-C18 column with phosphate buffer (pH 7.0) as water phase and methanol-acetonitrile (2 ∶ 1) as organic phase (gradicnt elution) at the flow rate of 1.0 rnl/min.The column temperature was 40 ℃,and detection wavelength was set at 262 nm.RESULTS:The separation rates of hydrochlorothiazide and candesartan cilexetil were all more than 1.5.The linear ranges were 2.5-100.0 mg/L for hydrochlorothiazide and 1.62-64.8 mg/L for candesartan cilexetil (all r=0.999 9).The mean recoveries were 99.23％ (RSD=1.23％,n=9) and 99.78％ (RSD=0.84％,n=9),respectively.CONCLUSIONS:2 kinds of main components are well-separated by this method.The method is accurate and reliable,and suitable for simultaneous determination of 2 kinds of main components in Compound hydrochlorothiazide/candesartan cilexetil tablets.关键词
坎地沙坦酯/氢氯噻嗪/片剂/含量测定/高效液相色谱法Key words
Candesartan cilexetil/ Hydrochlorothiazide/ Tablets/ Content determination/ HPLC分类
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谢鹏,王敏..HPLC法同时测定复方坎地沙坦酯氢氯噻嗪片中的主成分含量[J].中国药房,2013,24(25):2372-2374,3.
