中国药理学通报2013，Vol.29Issue(7)：975-980,6.DOI:10.3969/j.issn.1001-1978.2013.07.020
高含量DHA/EPA甘油三酯促进肝脏糖原合成及其作用机制的研究
Effects of high-content DHA/EPA triglyceride on promoting hepatic glycogen synthesis
摘要
Abstract
Aim To investigate the effects of high-content DHA/EPA triglyceride on hepatic glycogen synthesis and its mechanisms in diabetic mouse. Methods The diabetic mouse model was developed by feeding high fat food. The fasting plasma glucose, oral glucose tolerance, serum insulin and hepatic glycogen were assayed after feeding high-content DHA/ EPA triglyceride for 15 weeks. The mRNA expression of key genes associated with PI3K/PKB/GSK-3β insulin signal transduction pathway was detected by reverse transcription polymerase chain reaction ( RT-PCT ). Results High-content DHA/EPA triglyceride significantly decreased the level of fasting plasma glucose, reduced serum insulinemia, improved oral glucose tolerance , and increased hepatic glycogen synthesis, compared with model group ( P <0. 01 ). Meanwhile, significantly enhanced mRNA expressions of InsR, IRS-2, PI3K and PKB, and decreased GSK-3β expression were found( P <0. 01 vs model control ). And the efficacy of high-content DHA/EPA triglyceride was the best of all three fish oils tested. Conclusion The high-content DHA/EPA triglyceride could increase the hepatic glycogen synthesis and regulate the blood glucose balance by activating the pivotal genes of PI3K/ PKB/GSK-3β insulin signal transduction pathway.关键词
高含量DHA/EPA甘油三酯/糖尿病/血糖/血清胰岛素/糖原合成/胰岛素信号通路Key words
high-content DHA/EPA triglyceride/ diabetes/ blood glucose/ serum insulin/ glycogen synthesis/ insulin signal pathway分类
医药卫生引用本文复制引用
朱昱哲,王静凤,詹麒平,孙兆敏,王玉明,薛长湖..高含量DHA/EPA甘油三酯促进肝脏糖原合成及其作用机制的研究 [J].中国药理学通报,2013,29(7):975-980,6.基金项目
"十二五"国家科技支撑计划(No 2012BAD33B07) （No 2012BAD33B07）
海洋公益性行业科研专项(No 201105029) （No 201105029）
泰山学者建设工程专项经费 （）
长江学者和创新团队发展计划资助 （）
