中国药理学通报2013，Vol.29Issue(7)：999-1003,5.DOI:10.3969/j.issn.1001-1978.2013.07.025
缝隙连接蛋白Cx43介导硫化氢后处理对大鼠心肌的保护作用
Protective effects of Cx43-mediated exogenous hydrogen sulfide postconditinoing on rat hearts
摘要
Abstract
Aim To investigate whether connexin 43 ( Cx43 ) mediated hydrogen sulfide postconditioning protects isolated rat hearts against ischemia/reperfusion ( I/R )injury. Methods 72 male SD rat hearts were i-solated and linked to the Langendorff apparatus. They were randomly divided into 6 groups ( n = 12 ): sham group( Sham ), ischemia /reperfusion group ( I /R ), DMSO group ( DMSO ), 18β-AGA group ( AGA ), hydrogen sulfide postconditioning group ( NP ), and hydrogen sulfide with 18β-AGA group( N + A ). The heart rate ( HR ) , the left ventricular diastolic pressure ( LVEDP ), the left ventricular developed pressure ( LVDP ), the maximum rate of increase or decrease of left ventricular pressure ( ± dp/dtmax ) were recorded at 20 min of equilibrium and 60 min of reperfusion respectively. Myocardial infarct size was measured by tri-phenyl tetrazolium chloride ( TTC ) staining. The expression of total Cx43 ( tCx43 ) and phosphorylated Cx43 ( pCx43 ) in mitochondria and cytosol were determined with Western blot analysis at the end of reperfusion. Results There were no differences in baseline hemodyamics observed among the experimental groups ( P > 0. 05 ). After reperfusion, compared with I/R group, NP group had better hemodynamics , the myocardial infarct size was much lower( P < 0. 05 ), the expression of tCx43 in mitochondria increased significantly , but decreased significantly in cytosol, the expression of pCx43 was same to tCx43. However, 18β-AGA abolished the cardioprotective effects offered by hydrogen sulfide postconditioning and decreased tCx43 and pCx43 expression in mitochondria( P <0. 05 ). Conclusion Exogenous hydrogen sulfide postconditioning effectively protects isolated rat hearts against ischemia and reperfusion injury via activating Cx43.关键词
硫化氢/后处理/缺血/再灌注损伤/心肌保护/线粒体/缝隙连接蛋白Cx43Key words
hydrogen sulfide/ postconditioning/ ischemia/reperfusion injury/ cardioprotection/ mitochondria/ connexin 43分类
医药卫生引用本文复制引用
季永,张可璇,毛洪雅,王平..缝隙连接蛋白Cx43介导硫化氢后处理对大鼠心肌的保护作用[J].中国药理学通报,2013,29(7):999-1003,5.基金项目
江苏省教育厅资助项目 (No 6KJD320186) （No 6KJD320186）
徐州医学院院长基金 (No 9KJZ02) （No 9KJZ02）
