中国油料作物学报2013，Vol.35Issue(3)：257-261,5.DOI:10.7505/j.issn.1007-9084.2013.03.005
珍珠豆型花生品种白沙1016核型分析
Karyotype analysis of Spanish peanut variety Baisha 1016
摘要
Abstract
Spanish peanut variety (Arachis hypogaea L.var.vulgaris Harz) Baisha 1016 was a popular cultivar and an elite germplasm resource.Using the highly conserved repeat sequences of 5S rDNA and 45S rDNA as probes,FISH (fluorescence in situ hybridization) analysis of metaphase chromosomes in cells from peanut root tips were conducted.Hybridization signals were observed in 12 chromosomes including in both long and short arms of one pair of chromosome,short arms of two pairs of chromosomes,and long arms of three pairs of chromosomes.Most of the chromosomes of Baisha 1016 could be distinguished and characterized based on the hybridization signals combined with DAPI+ bands and length,arm ratio and area of chromosomes.The results provided the genetic basis of chromosome analysis for utilization of Baisha 1016 in further breeding programs.关键词
白沙1016/45S rDNA/5S rDNA/荧光原位杂交(FISH)/核型分析Key words
Baisha 1016/45S rDNA/5S rDNA/FISH/Karyotype analysis分类
农业科技引用本文复制引用
杜培,张新友,李丽娜,黄冰艳,董文召,汤丰收..珍珠豆型花生品种白沙1016核型分析[J].中国油料作物学报,2013,35(3):257-261,5.基金项目
国家花生产业技术体系建设项目(CARS-14) （CARS-14）
国家科技支撑计划(2011BAD35B04) （2011BAD35B04）
