中国药业2013，Vol.22Issue(11)：4-5,2.
高效液相色谱法测定月季花中金丝桃苷含量
Determination of Hyperin Content in Rosa chinensisJacq by HPLC
崔慧芳 1常欣1
作者信息
- 1. 浙江省宁波市药品检验所,浙江宁波315048
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determining the hyperin content in Rosa chinensis Jacq.Methods The Agilent C18 column(100 mm×4.6 mm,2.7 μm) was adopted with the mobile of acetonitrile-0.05％ phosphoric acid(16:84); the detection wavelength was set at 360 nm with flow rate of 0.5 mL/min.Results A good linearity relation was observed between the sample size of hyperin during the range of 0.047 68-0.476 80 μg with the peak area.The average recovery rate of hyperin was 97.22％,RSD =0.89％ (n=9).Conclusion This method is rapid,convenient,stable,reliable and strongly specific for the dermination of hyperin in Rosa chinensis Jacq.关键词
高效液相色谱法/月季花/金丝桃苷/含量测定Key words
HPLC/ rosa chinensis Jacq/ hyperin/ content determination分类
医药卫生引用本文复制引用
崔慧芳,常欣..高效液相色谱法测定月季花中金丝桃苷含量[J].中国药业,2013,22(11):4-5,2.
