中国药业2013，Vol.22Issue(11)：8-10,3.
高效液相色谱荧光法测定人血浆中奥美沙坦浓度
Determination of Olmesartan Concentration in Human Plasma by HPLC Fluorescence Method
封宇飞 1倪明月 2孙春华1
作者信息
- 1. 卫生部北京医院药学部,北京 100730
- 2. 北京大学药学院,北京 100083
- 折叠
摘要
Abstract
Objective To establish a HPLC method for the determination of olmesartan in human plasma.Methods Prazosin hydrochloride was used as the internal standard.The plasma sample adopted by the precipittion method.The Apollo C18 column (150mm×4.6 mm,5 μm) was used as the analytical column with the mobile phase of 0.1％ triethylamine (adjusting pH to 2.5 with formic acid)-acetomitrile (75:25).The excitation wavelength(λex) of 260 nm and emission wavelength of (λem) 379 nm.The flow rate was 1.0 mL/min.The column temperature was 25 ℃.Results A good linear relation was obtained within the plasma olmesartan concentration range of 10.00-1 280.00 ng/mL(r =0.999 6).The lowest detection concentration was 10.00 ng/mL,the relative recovery rate was 97.53％-99.24％ (n =5) and the absolute recovery rate was 86.89％-89.32％ (n =5); the within-day RSD and the between-day RSD were 1.25％-2.26％ and 1.14％-5.55％ respectively.Conclusion This method is shown to be simple,accurate and sensitive with strong specificity and good reproducibility,and can be used for the determination of olmesartan level in human plasma and the pharmacokinetic study of olmesartan.关键词
奥美沙坦/高效液相色谱荧光法/血药浓度Key words
olmesartan/ HPLC-fluorescence method/ blood drug concentration分类
医药卫生引用本文复制引用
封宇飞,倪明月,孙春华..高效液相色谱荧光法测定人血浆中奥美沙坦浓度[J].中国药业,2013,22(11):8-10,3.
