中国药业2013，Vol.22Issue(11)：43-44,2.
高效液相色谱法测定尪痹片中芍药苷含量
Determination of Paeoniflorin Content in Wangbi Tablet by HPLC
李玉英 1吴俊贤 1郑惠 2赵琪琪3
作者信息
- 1. 广东省河源市食品药品检验所,广东河源517000
- 2. 广东医学院,广东东莞 523808
- 3. 广州中医药大学,广东广州 510006
- 折叠
摘要
Abstract
Objective To establish a method for the content determination of paeoniflorin in Wangbi Tablet.Methods The HPLC was performed on the Synergi 4u Fusion-RP 80A column(150 mm ×4.60 mm,4 μm) with the mobile phase of acetonitrile-methanol-1％ phosphoric acid (9.3∶4.7∶86); the flow rate was 1.0 mL/min; the column temperature was 30℃; the detection wavelength was 228 nm.With 50％ methanol as solvent,paeoniflorin in Wangbi Tablet was extracted by ultrasound for 30 min.Results The sample size of paeoniflorin during the range of 0.120-2.034 ng showed a good linear relation with the peak area.The regression equation was Y=1142.84709X+0.504 66,r=0.999 82(n=6),the average recovery rate was 97.27％,RSD 1.80％ (n=6).Conclusion This method can precisely determine the paeoniflorin content in Wangbi Tablet.关键词
尪痹片/芍药苷/含量/高效液相色谱法Key words
Wangbi Tablet/ paeoniflorin/ content/ HPLC分类
医药卫生引用本文复制引用
李玉英,吴俊贤,郑惠,赵琪琪..高效液相色谱法测定尪痹片中芍药苷含量[J].中国药业,2013,22(11):43-44,2.
