中国肿瘤生物治疗杂志2013，Vol.20Issue(3)：312-316,5.DOI:10.3872/j.issn.1007-385X.2013.03.010
腺病毒介导miRNA-29a过表达抑制人胃癌细胞的增殖
Adenovirus mediated over-expression of miRNA-29a inhibits proliferation of human gastric cancer cells
摘要
Abstract
Objective:To explore the inhibitory effect of miRNA-29a(miR-29a) on the proliferation of human gastric cancer cell lines SGC-7901 and AGS through over-expression of miR-29a mediated by the recombinant replication-deficient human adenovirus type 5 vector.Methods:The recombinant adenovirus Ad-miR-29a containing pre-miR-29a or control adenovirus Ad-LacZ containing LacZ gene was constructed and infected into human gastric cancer SGC-7901 and AGS cells,respectively.The expressions of miR-29a in SGC-7901 and AGS cells were detected by real-time PCR.CCK-8 assay was employed to examine the inhibitory effect of miR-29a on the proliferation of human gastric cancer cell lines.The flow cytometry assay was used to analyze cell cycle of SGC-7901 and AGS cells.The migration ablilities of SGC-7901 and AGS cells were assessed by Transwell assay.Results:Compared with the Ad-LacZ group,the Ad-miR29a group expressed higher level of miR-29a in both SGC-7901 and AGS cells ([17.35 ± 0.71] vs [1.12 ± 0.09],[26.50 ±1.09] vs [0.95 ± 0.04],P＜0.01).The proliferation of SGC-7901 and AGS was significantly inhibited in the Ad-miR29a group as compared with that in the Ad-LacZ group.Six days after Ad-miR29a infection,the D value was significantly decreased ([0.54± 0.03] vs [0.77 ± 0.04],[0.70 ± 0.03] vs [0.88 ± 0.04],P＜0.01).Theproliferation of cells in the Ad-LacZ group showed no significant changes as compared with that in the uninfected group (P ＞0.05).Meanwhile,The percentage of SGC-7901 or AGS cells arrested in G0/Gt period in the Ad-miR29a group was significantly higher than that in the Ad-LacZ group ([63.10 ± 4.91] ％ vs [47.60 ± 5.31] ％,[69.80 ± 3.15] ％ vs [54.60 ± 4.22] ％,P ＜ 0.05].However,no significant changes were found in the migration ability of gastric cancer SGC-7901 and AGS cells.Conclusion:miR-29a can effectively suppress the proliferation of human gastric cancer cells,which makes a promising new therapeutic target for gastric cancer.关键词
miRNA-29a/腺病毒/胃癌/增殖/细胞周期Key words
miRNA-29a(miR-29a)/adenovirus/ gastric cancer/ proliferation/ cell cycle分类
医药卫生引用本文复制引用
刘志鹏,王宗华,卢斌,胡春燕,李学成,邹利全,张方征,陈陵..腺病毒介导miRNA-29a过表达抑制人胃癌细胞的增殖[J].中国肿瘤生物治疗杂志,2013,20(3):312-316,5.基金项目
重庆市自然科学基金资助项目(No.CSTC,2010BB5158,No.CSTC,2011AC5024,No.CSTC,2011jjA0878,No.CSTC,2011jjA0882). Project supported by the Natural Science Foundation of Chongqing (No.CSTC,2010BB5158,No.CSTC,2011 AC5024,No.CSTC,2011jjA0878,No.CSTC,2011jjA0882) （No.CSTC,2010BB5158,No.CSTC,2011AC5024,No.CSTC,2011jjA0878,No.CSTC,2011jjA0882）
