中山大学学报（医学科学版）2013，Vol.34Issue(3)：364-370,7.
EGCG对人肝癌细胞株HepG2裸鼠移植瘤COX-2、VEGF和bFGF表达的调控
Regulation of EGCG on Expression of COX-2, VEGF, and bFGF in Nude Mice Xenogrfts Tumor of Human HepG2 Cells
摘要
Abstract
[Objective] To investigate the inhibitory effects of (-)-Epigallocatechin-3-gallate (EGCG) on growth and tumor angiogenesis and regulations of EGCG on expression of COX-2 (cyclooxygenase-2),VEGF (vascular endothelial growth factor),and bFGF (basic fibroblast growth factor) in nude mice xenogrfts tumor of Human HepG2 Cells.[Methods] HepG2 cells were transplanted into the dorsal subcutaneous tissue of athymic nude mice.The mice were treated with different concentrations of EGCG 10 days after transplantation,and were killed after 38 days.Tumor volume and weight were measured.Expression of COX-2,VEGF,and bFGF were detected by indirect immunofluorescence flow cytometry assay and Western blot and microvessel density (MVD) using immunohistochemical methods.[Results] The average tumor volume was significantly smaller and the average tumor weight was significandy lighter in EGCG 50mg/kg group than in control group [(1174.6 ± 793.5)mm3 vs (649.3 ± 100.8) mm3 and (0.572 ± 0.138)g vs (0.340 ± 0.066)g,P ＜ 0.05].The inhibitory rate of tumor growth was 40.56％.MVD values of the saline control group and the group of EGCG 50 mg/kg were (23.24 ± 1.76) and (14.27 ± 1.25),the difference was statistically significant (P ＜ 0.01);indirect immunofluorescence flow cytometry and Western Blot analysis results suggest that EGCG was concentration-dependently inhibited HepG2 cells transplanted tumors in the expression of COX-2,VEGF and bFGF.[Conclusions]EGCG inhibits the growth and angiogenesis of HepG2 cell xenogrfts in small nude mice.It's mechanism may be related to the inhibition of COX-2 expression and the resulting VEGF and bFGF protein expression lower them.关键词
HepG2细胞/裸鼠移植瘤/新生血管生成/COX-2/VEGF/bFGFKey words
HepG2 cells/ xenogrfts/ angiogenesis/ COX-2/ VEGF/ bFGF分类
医药卫生引用本文复制引用
周薇,戴奇刚,邓鑫,荣孪君,王英桂..EGCG对人肝癌细胞株HepG2裸鼠移植瘤COX-2、VEGF和bFGF表达的调控[J].中山大学学报（医学科学版）,2013,34(3):364-370,7.基金项目
广西壮族自治区科学技术厅青年基金(桂科青0832056) （桂科青0832056）
