中山大学学报（医学科学版）2013，Vol.34Issue(3)：387-396,10.
PKC及蛋白酪氨酸磷酸化信号通路对血管平滑肌细胞表型转化过程中容积调节性氯通道的作用
Regulation of PKC and Protein Tyrosine Phosphorylation on VRCC During the Phenotype Modulation of Femoral Artery and Vein Smooth Muscle cells
摘要
Abstract
[Objective] To compare the differences between the effects of protein kinase C (PKC) and protein tyrosine phosphorylation on volume-regulated chloride channel (VRCC) during the phenotype modulation of smooth muscle cells in artery and vein.[Methods] We used the whole cell patch clamp to evaluate swelling induced Cl-currents (ICl.vol) in primay,Ⅳ and Ⅷ passage rat femoral artery (FASMC) and vein smooth muscle cells (FVSMC) ; and the regulative the effects of protein tyrosine kinase (PTK) inhibitor genistein,protein tyrosine phosphatase (PTP) inhibitor sodium orthovanadate,and PKC activator PDBu on VRCC activity in these cells were examined.[Results] Hypotonic solution induced larger ICl.vol in FASMC than in FVSMC.PTK inhibitor genistein and PKC activator PDBu inhibited VRCC activity in both FASMC and FVSMC,and this inhibitory effect was gradually enhanced in the cells from primary to passage Ⅷ.Whereas PTP inhibitor sodium orthovanadate increased VRCC activity in both FASMC and FVSMC,and this increase was gradually decreased as cell passages increased.Moreover,at the same cell passage,the regulatory effects of genistein,PDBu,and sodium orthovanadate on VRCC activity were more prominent in FVSMC than in FASMC.[Conclusion] PTK,PTP,and PKC could regulate VRCC activity,which were more prominent in FVSMC than in FASMC.Moreover,VRCC activity and the regulatory effects of PKC and protein tyrosine phosphorylation on VRCC were gradually increased as vascular cells switch from contractile to proliferative phenotypes.关键词
容积调节性氯通道(VRCC)/动脉和静脉/表型转化/血管平滑肌细胞Key words
volume-regulated chloride channel (VRCC) / artery and vein/ phenotype modulation/ vascular smooth muscle cells分类
医药卫生引用本文复制引用
张敏,吕晓飞,刘婕,关永源,杜艳华..PKC及蛋白酪氨酸磷酸化信号通路对血管平滑肌细胞表型转化过程中容积调节性氯通道的作用[J].中山大学学报（医学科学版）,2013,34(3):387-396,10.基金项目
国家自然科学基金(81230082 （）
81173055 （）
30900578) （）
广州市科技计划项目(2011J2200077). （2011J2200077）
