中山大学学报（医学科学版）2013，Vol.34Issue(3)：434-437,4.
混合抗体型自身免疫性溶血性贫血的血型定型和抗体鉴定
Blood Group Typing Test and Antibody Identification of Autoimmune Hemolytic Anemia with both Warm and Cold Autoantibodies
摘要
Abstract
[Objective] To resolve the difficulty in blood group typing test and antibody identification test of autoimmune hemolytic anemia (AIHA) with both warm and cold autoantibodies.[Methods] Patient's RBC was used for ABO and rhesus typing test after it had been eluted with gently heat and chloroquine.The ABO reverse typing test was performed after the autoantibodies in patient's plasma had been absorbed with O group blood cell.Polyethylene glycol (PEG) adsorption method and auto-adsorption method were studied by comparing the total time and numbers of the adsorption.Antibodies were identified with ethylether elute.[Results] ABO and Rh blood group were typed correctly in 21 and 15 cases,respectively.Seven cases were detected to have both allo-antibody and auo-antibody,including anti-S,anti-Ce,anti-c and anti-E,however,antibodies specificity had not been identified in 3 cases.Fourteen cases had only autoantibody.The mean time required by PEG adsorption and auto-adsorption for autoantibody removal was 39.3 minutes (total,825 minutes) and 98.7 minutes (total,2 070 minutes).The mean number of autoadsorption for emoving autoantibody were 2.6 (total,55) and 3.1 (total,66).[Conclusion] Gently heat elute combines chloroquine elute is a good choice to perform blood group typing test for AIHA patients with both cold and warm autoantibodies.PEG method is a rapid,simple and effective way to remove autoantibody and detect underlying alloantibody,which can be used in routine practice.关键词
自身免疫性溶血性贫血/放散/吸收/氯喹/聚乙二醇Key words
autoimmune hemolytic anemia/ elution/ adsorption/ chloroquine/ polyethylene glycol分类
医药卫生引用本文复制引用
骆宏,张润青,罗广平,赵阳,魏玲,莫春妍,姬艳丽,王贞..混合抗体型自身免疫性溶血性贫血的血型定型和抗体鉴定[J].中山大学学报（医学科学版）,2013,34(3):434-437,4.基金项目
广州市医药卫生科技项目(201102A213233) （201102A213233）
