作物研究2013，Vol.27Issue(3)：224-228,5.DOI:10.3969/j.issn.1001-5280.2013.03.04
番茄突变体jai1-1高效筛选—再生体系的建立
Establishment of High Efficient Screening and Regeneration System of Tomato mutant jai1-1 in Vitro
摘要
Abstract
Tomato(Lycopersicon esculentum Mill.)wild type JAIl and mutant jail-1 screened by different concentrations of MeJA were used as the materials in the study.The cotyledon and hypocotyl of wild type JAI1 and mutant jai1-1 were cultured in vitro to research the effects of genetype and different hormone combinations on tomato callus induction,adventition bud differentiation and rooting,and then identified the results with PCR reaction.The results show that 1/2 MS + MeJA 50 μM/L was the proper media to screen the mutant jai1-1,and that MS + ZT 1.0 mg/L + IAA 0.1 mg/L and MS + ZT 2.0 mg/L + IAA 0.2 mg/L were the best media for adventition bud differentiation for JAI1 and jai1-1 respectively.MS + 6-BA 2.0 mg/L + IAA 0.2 mg/L and 1/2MS + NAA 0.1 mg/L were the best media for callus induction and root induction for both JAI1 and jai1-1,respectively.关键词
番茄/组织培养/愈伤组织/再生体系Key words
Tomato/Tissue culture/Callus/Regeneration system分类
生物科学引用本文复制引用
范锡麟,王少岭,肖应辉,王志龙..番茄突变体jai1-1高效筛选—再生体系的建立[J].作物研究,2013,27(3):224-228,5.基金项目
国家自然科学基金项目(31071674). （31071674）
