植物学报2013，Vol.48Issue(3)：344-353,10.DOI:10.3724/SP.J.1259.2013.00344
降解组测序技术在植物miRNA研究中的应用
Use of Degradome Sequencing in Study of Plant MicroRNAs
摘要
Abstract
MicroRNA (miRNA) chip and sequencing have been used to detect miRNAs in species.With the discovery of more miRNAs,miRNA target genes are the key to study miRNA biological function.Finding miRNA target genes mainly relies on computational prediction,AGO protein co-immunoprecipitation and luciferase.Deep sequencing,or degradome sequencing,can be used for high-throughput identification of targets of miRNAs.The method includes deep sequencing,bioinformatic analysis and 5'-repid amplification of cDNA ends.It has been successfully used for global identification of miRNA-target RNA pairs in Arabidopsis thaliana,Oryza sativa and Physcomitrella patens.This paper reviews research progress in degradome sequencing in plant miRNA target study and discusses trends in further application of degradome sequencing.关键词
降解组测序/高通量测序/miRNA/靶基因Key words
degradome sequencing/ high-throughput sequencing/ miRNA/ target gene引用本文复制引用
董淼,黄越,陈文铎,徐涛,郎秋蕾..降解组测序技术在植物miRNA研究中的应用[J].植物学报,2013,48(3):344-353,10.基金项目
国家自然科学基金(No.30772712) （No.30772712）
