中国中西医结合杂志2013，Vol.33Issue(6)：789-794,6.
乌附经方预处理对大鼠缺血再灌注心肌细胞凋亡的影响
Effects of Preconditioning of Wufu Jingfeng on Ischemia/Reperfusion Injury Induced Apoptosis in Rats
摘要
Abstract
Objective To observe the protective effect of Wufu Jingfang (W J,containingAconitum carmichaeli Debx,Radix Aconiti Lateralis Preparatae,Rhizoma Pinelliae,and snakegourd fruit) on myocardial ischemia-reperfusion injury (I/R) of rats,thus exploring the feasibility of recipes containing eighteen imcompatible pairs for specific pathological conditions.Methods Fifty male Wistar rats were randomly divided into five groups,i.e.,the sham-operative control group (the SH group),the I/R group,the low dose WJ I/R group (the I/R +JFL group),the middle dose WJ I/R group (the I/R +JFM group),the high dose WJ I/R group (the I/R +JFH group),10 in each group.Rats in the latter three groups were administered with WJ at 0.75 mL/100 g,1.50 mL/100 g,and 3.00 mL/100 g body weight for 14 consecutive days by gastrogavage.All groups except the SH group received ligation of left anterior descending branch of coronary artery for 30-min ischemia followed by 120-min reperfusion.The micro-structural changes of myocardial mitochondria were observed by transmission electron microscope.The ischemic cardiomyocyte apoptosis was detected in each group using one-step terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL).The mRNA expressions of B-cell leukemia/lymphoma 2 (Bcl-2) and Bcl2 associated x protein (Bax) were detected by RT-PCR.The activities of lactic dehydrogenase (LDH) and creatine kinase (CK) were detected using ELISA.The myocardial infarct size was detected.Results Compared with the I/R group,WJ pretreatment significantly suppressed the release of LDH and CK (Besides,the release of LDH and CK reduced along with increased dose.),reduced the myocardial infarct size,and lowered myocardial apoptosis index (P ＜0.05).WJ pretreatment also modulated Bcl-2/Bax ratio by up-regulating Bcl-2 expression level while decreasing Bax expression level.Conclusions WJ pretreatment might protect the heart from I/R injury via decreasing myocardial cell apoptosis.The results suggested that eighteen incompatible pairs is not absolute,but relative.Chinese medical preparation containing opposite Chinese herbs could be used in specific pathological states such as ischemic cardiomyopathy.关键词
乌头/附子/半夏/栝楼/十八反/缺血再灌注/细胞凋亡Key words
Aconitum carmichaeli Debx/ Radix Aconiti Lateralis Preparata/ Rhizoma Pinellia/snakegourd fruit/ eighteen imcompatible pairs / ischemia/reperfusion/ apoptosis引用本文复制引用
李玲,马瑜红,王素青..乌附经方预处理对大鼠缺血再灌注心肌细胞凋亡的影响[J].中国中西医结合杂志,2013,33(6):789-794,6.基金项目
南阳市2009年科技攻关计划项目(No.2009GG039) （No.2009GG039）
