中国中西医结合杂志2013，Vol.33Issue(6)：830-833,4.
赤芍总苷对D-半乳糖诱导衰老大鼠脑组织TOLL受体和IL-33的实验研究
Effects of Total Paeony Glucosides on mRNA Expressions of Toll Receptors and Interleukin-33 in the Brain Tissue of D-galactose Induced Aging Rats: an Experimental Research
摘要
Abstract
Objective To investigate effects of total paeony glucosides (TPGs) on the expressions of Toll receptors (TLR4) and interleukin-33 (IL-33) in the brain tissue of D-galactose-induced aging rats.Methods Fifty SD rats were randomly divided into 5 groups,i.e.,the blank control group,the model group,the high dose TPG group,the middle dose TPG group,and the low dose TPG group,10 in each group.Equal volume of normal saline was subcutaneously injected to rats in the blank control group,while 10％ D-galactose was subcutaneously injected to rats in the rest groups at 0.125 mL/g,once a day for 8 successive weeks to induce the aging rat model.TPG was administered at 300 mg/kg,150 mg/kg,and 75 mg/kg to rats in the high,middle,and low dose TPG groups while injecting D-galactose from the 5th week of model preparation,once daily for 4 successive weeks.Equal volume of normal saline was administered to rats in the blank control group and the model group,once daily.The capability for learning and memory was detected using Morris water.The mRNA expressions of TLR4 and IL-33 in the brain tissue were detected using ELISA.Results Compared with the blank control group,the capability for learning and memory decreased in the model group with statistical difference (,P ＜0.05).Compared with the model group,the capability for learning and memory was obviously improved in all the medicated groups in a dose-dependent manner,showing statistical difference (P ＜0.05).Compared with the blank control group,mRNA expressions of TLR4 and IL-33 in the brain tissue obviously increased after medication in the model group,showing statistical difference (P ＜0.05).Compared with the model group,mRNA expressions of TLR4 and IL-33 in the brain tissue obviously decreased after medication in all the medicated groups in a dose-dependent manner,showing statistical difference (P ＜0.05).Conclusion TPGs improved D-galactose induced aging rats' capability for learning and memory through regulating changes of TLR4 and IL-33 expressions.关键词
赤芍总苷/衰老大鼠/TOLL受体/白细胞介素-33Key words
total paeony glycoside/ aging rat/ TOLL receptor/ interleukin-33引用本文复制引用
张海燕,刘忠锦,陈志伟..赤芍总苷对D-半乳糖诱导衰老大鼠脑组织TOLL受体和IL-33的实验研究[J].中国中西医结合杂志,2013,33(6):830-833,4.基金项目
黑龙江省教育厅科学技术研究资助项目(No.12521652) （No.12521652）
