中医药学报2013，Vol.41Issue(3)：43-47,5.
星点设计-响应面法优选芪玄抑甲宁的提取工艺
Optimization of Extraction Technology for Qixuan Yijianing by Central Composite Design and Response Surface Methodology
摘要
Abstract
Objective:To optimize extraction technology of Qixuan Yijianing.Methods:With soaking time,the amount of water,extraction time and extraction times as independent variables,yields of calycosin-7-0-β-D-glycoside,harpagide and rosmarinic acid as dependent variable,multiple linear regression and binomial of each level of independent variableswere fitted.Optimum technology was selected by central composite design and response surface methodology,and prediction was carried out by comparing the observed and predicted values.Results:Optimum extraction technology parameters were as follows:soaking time 74.4rain,the amount of water 13.56-fold,extraction time 1.165h and extraction times twice.Under this condition,maximum estimated value of the sum of calycosin-7-O-β-D-glycoside,harpagide and rosmarinic acid was 0.36,which was agreed with model predicted value.Conclusion:Optimized extraction technology is simple,reliable and highly predictive.关键词
芪玄抑甲宁/星点设计/响应面法Key words
Qixuan Yijianing/ Central composite design/ Response surface methodology分类
医药卫生引用本文复制引用
柳长凤,苗晶囡,邱军强,崔晓旭,刘树民..星点设计-响应面法优选芪玄抑甲宁的提取工艺[J].中医药学报,2013,41(3):43-47,5.基金项目
黑龙江省青年科学基金(No.QC2012C038) （No.QC2012C038）
黑龙江中医药大学科研基金(No.B201104) （No.B201104）
黑龙江省研究生创新科研项目(No.YJSCX2012-356HLJ) （No.YJSCX2012-356HLJ）
