分析化学2013，Vol.41Issue(6)：893-898,6.DOI:10.3724/SP.J.1096.2013.20701
高效液相色谱和液相色谱-电喷雾质谱联用法鉴定辛硫磷在鲫鱼肝微粒体中代谢产物及途径
Identification of Metabolites and Pathway in Liver Microsomes of Phoxim in Crucian Carp by High Performance Liquid Chromatography and Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry
摘要
Abstract
The liver microsomes were prepared by differential ultracentrifugation for the investigation of in vitro metabolism of phoxim.The metabolites were extracted twice from the reaction solution with ethyl acetate,remove the organic phase to rotary evaporator by near dryness at 40 ℃,then constant volume by 1 mL methanol,separated and determined by HPLC,and then identified by HPLC-MS (ESI).Three major metabolites (M1:O,O,O',O'-tetraethyldithiopyrophosphate,M2:diethylthiophosphate and Ms:phoxom) indicated that phoxim underwent desulfur-oxidation and hydrolysis in crucian carp liver microsomes.Therefore,this study deduced the metabolic pathway and metabolic characteristic of phoxim in liver microsomes of crucian carp and laid a solid foundation for further study of its metabolism and metabolic enzymes.关键词
辛硫磷/鲫鱼/肝微粒体/体外代谢/液相色谱-电喷雾质谱联用Key words
Phoxim/Crucian carp/Liver microsomes/Vitro metabolism/Liquid chromatography-electrospray ionization mass spectrometry引用本文复制引用
刘荣飞,刘幸,刘晓宇,范雯婷,赵冬冬,石旺荣,朱雨田,李宏..高效液相色谱和液相色谱-电喷雾质谱联用法鉴定辛硫磷在鲫鱼肝微粒体中代谢产物及途径[J].分析化学,2013,41(6):893-898,6.基金项目
本文系国家自然科学基金(No.31171694)和湖北省自然科学基金(No.2009CDB410)资助项目 （No.31171694）
