分析化学2013，Vol.41Issue(6)：899-904,6.DOI:10.3724/SP.J.1096.2013.21050
超高效液相色谱-串联质谱法同时测定绵羊唾液中14种β-受体激动剂
Simultaneous Determination of 14 Kinds of β-Agonist Residues in Sheep Saliva by Ultra Performance Liquid Chramatogrephy-Tandem Mass Spectrometry
摘要
Abstract
A sensitive method was developed for the determination of 14 kinds of β-agonists residues in sheep saliva by solid phase extraction pretreatment and ultra performance liquid chromatography tandem mass spectrometry.The sample was adjusted by pH5.2 ammonium acetate buffer and centrifuged separation to eliminate impurity,then passed through MCX cartridge to clear-up.The test potion was separated by waters BEH C18 columns with gradient elution program.Analyte confirmation was performed using multiple reaction monitoring (MRM) with one precursor ion and productions as identifiers.The results were calculated by internal standard method.The LODs were 0.1-0.2 μg/L,LOQs were 0.3-0.5 μg/L,the recoveries were in range of 60％-110％,and the relative standard deviations (RSDs) were less than 20％ at three spiked levels for β-agonists.关键词
β-受体激动剂/唾液/固相萃取/超高效液相色谱-串联质谱Key words
β-Agonist/Saliva/Solid phase extraction/Ultra performance liquid chromatography-tandem mass spectrometry引用本文复制引用
李阳,苏晓鸥,王瑞国,王培龙,张维,程劼..超高效液相色谱-串联质谱法同时测定绵羊唾液中14种β-受体激动剂[J].分析化学,2013,41(6):899-904,6.基金项目
本文系公益性行业(农业)科研专项(No.201203088)资助 （农业）
