分析化学2013，Vol.41Issue(6)：911-916,6.DOI:10.3724/SP.J.1096.2013.20897
衍生化毛细管气相色谱法同时测定无糖食品中的多种糖醇类甜味剂
Determination of Sugar Alcohols Sweeteners in Sugar-free Food by Derivatization Capillary Gas Chromatography
张雅珩 1周围 1李斌1
作者信息
- 1. 甘肃出入境检验检疫局检验检疫综合技术中心,兰州730010
- 折叠
摘要
Abstract
A capillary gas chromatographic method for simultaneous determination of sugar alcohol sweeteners in sugar-free food was set up.The sugar alcohols were extracted from samples with water.Then these extracts were purified,dried and derived to sugar alcohol acetates in the presence of pyridine and acetic anhydride (1 ∶ 1,V/V) at the temperature of 90 ℃ for 20 min.Furthermore the derivation reaction products were separated by gas chromatography with the OV-101 (50 m×0.25 mm×O.33 μm) capillary column,then determined by flame ionization detector and characterized by gas chromatography-mass spectrometry.The derivatives of erythritol,xylitol,mannitol,sorbitol and maltitol could be effectively separated from common sugars on the optimum chromatographic condition.The linear regression equations between the concentrations of the sugar alcohols and their peak areas of the derivatives were established in the range of 0.01-1.6 g/L.The correlation coefficients were 0.9995-0.9999.The average recovery was in the range of 74.6％-113.4％.The relative standard deviation is in the range of 2.4％-8.6％.The results demonstrated that the method had the advantages such as simplicity,rapid speed and reliability.The detection limits and recoveries were suitable for the determination of sugar alcohols sweeteners in sugar-free food.关键词
气相色谱/糖醇/衍生化/无糖食品Key words
Gas chromatography/Sugar alcohols/Derivatization/Sugar-free food引用本文复制引用
张雅珩,周围,李斌..衍生化毛细管气相色谱法同时测定无糖食品中的多种糖醇类甜味剂[J].分析化学,2013,41(6):911-916,6.
