分析化学2013，Vol.41Issue(6)：931-935,5.DOI:10.3724/SP.J.1096.2013.20578
超高效液相色谱法同时测定化妆品中的19种喹诺酮类抗生素
Determination of 19 Quinolone Antibiotics in Cosmetics by Ultra Performance Liquid Chromatography
摘要
Abstract
An ultra-liquid chromatographic (UPLC) method has been developed for the simultaneous determination of 19 quinolone antibiotics in cosmetics,ofloxacin,norfloxacin,ciproxacin,bifloxacin,sarafloxacin,oxolinic acid and flumequin etc.Different forms of cosmetic samples were prepared by ultrasonic extraction.The extraction solutions were obtained by high-speed centrifugation,the supematant was degreased by hexane,and then was filtered by 0.22 μm millipore filter,and the continued filtrate was taken for analysis.A Waters reversed phase column,ACQUITY UPLC BEH Shield RP C1s(100 mm×2.1 mm,1.7 μm) was used with methanol:acetonitrile (85 ∶ 15,V/V)-0.05 mol/L phosphate buffer(pH =3.6) as the mobile phase under the condition of gradient elution.And the diode array detector was used for the determination.The relative standard deviations (RSD) were 0.4％-1.1％ ; recoveries were 75.5％-105.1％ ; a variety of quinolones showed good linearity within the concentration range of 5-100 mg/L,correlation coefficients were greater than 0.996 ; detection limits were 1.2-14.7 mg/kg.This method has been successfully used for the determination of quinolone antibiotic in cosmetics products.关键词
超高效液相色谱法/喹诺酮类抗生素/化妆品Key words
Ultra performance liquid chromatography/Quinolone antibiotic/Cosmetics引用本文复制引用
陈静,郑荣,季申,王柯..超高效液相色谱法同时测定化妆品中的19种喹诺酮类抗生素[J].分析化学,2013,41(6):931-935,5.
