湖泊科学2013，Vol.25Issue(4)：551-557,7.
铜胁迫对菹草无菌苗多胺及活性氧代谢的影响
Effect of copper stress on polyamine and reactive oxygen species metabolism in aseptic seedlings of Potamogeton crispus L.
摘要
Abstract
Taking aseptic seedlings of P.crispus as the experimental material,the effect of different concentrations of Cu2 + (0,1,2,4,8,12 μmol/L) on the metabolism of polyamine(PAs) and reactive oxygen were investigated.With the increasing of Cu2+concentration,the results indicated that:(1) Superoxide dismutase (SOD) activity increased markedly,while the activities of peroxidase(POD) and catalase(CAT) increased progressively followed by a decline.(2) The generation rate of O2·-and the content of H2O2 and MDA increased,while soluble protein content decreased.(3) Free putrescine(Put) content first enhanced and then reduced notably.However,the content of free spermidine(Spd) decreased gradually and spermine(Spm) increased significantly.Therefore,the ratio of free (Spd + Spm)/Put promptly increased under high concentrations of Cu2 + stress.The changes of total PAs content were similar to those on free PAs.Perchloric acid soluble conjugated (PS-conjugated) Put and Spm changed in the same pattern as free PAs.While PS-conjugated Spd content increased slightly.In addition,the content of perchloric acid insoluble bound (PIS-bound) Put and Spd first increased and then decreased,but they were still higher than those in the control.Moreover,PIS-bound Spm content declined.(4) With the increase of Cu2 + concentration,the activity of arginine decarboxylase (ADC) sharply decreased after a transient increase,while the activity of ornithine decarboxylase(ODC) did not change significantly.Polyamine oxidase(PAO) and diamine oxidase(DAO) activities increased gradually,followed by a decrease.Our results suggested that Cu2 + stress induced obvious oxidative damage on aseptic seedlings of P.crispus.The up-regulation of antioxidant enzymes activities and Spm content,the changes of Put into Spd and Spm as well as the conversion between different forms of polyamines played a key role in the adaptation mechanism of aseptic seedlings of P.crispus under Cu2 + stress.关键词
菹草/无菌苗/Cu2+胁迫/活性氧/多胺代谢Key words
Potamogeton crispus L./aseptic seedling/Cu2+ stress/reactive oxygen/polyamine metabolism引用本文复制引用
姜岩,施国新,陈霖,汪鹏合,乔绪强,田秀丽..铜胁迫对菹草无菌苗多胺及活性氧代谢的影响[J].湖泊科学,2013,25(4):551-557,7.基金项目
江苏高校优势学科建设工程项目(164320H106)和国家自然科学基金项目(30870139)联合资助. （164320H106）
