渔业科学进展2013，Vol.34Issue(3)：61-67,7.
半滑舌鳎膜孕激素受体基因克隆与组织表达分析
Molecular cloning and tissue expression analysis of membrane progestin receptor alpha gene (mPRα) from half smooth tongue sole Cynoglossus semilaevis Günther
摘要
Abstract
Full-length cDNA encoding membrane progestin receptor alpha gene (mPRα)was firstly cloned from half smooth tongue sole Cynoglossus semilaevis Günther by homology cloning and RACE-PCR analysis.The length of complete cDNA sequence of mPRα gene was 1 319 bp.Sequence alignment of deduced amino acid of tongue sole mPRα and amino acid of other species showed that there were seven transmembrane domains.The rooted phylogenetic tree was constructed by the neighbor-joining method of MEGA 4.0,and the identity of tongue sole mPRα with other representative sequences was analyzed by ClustalX.The results indicated that the tongue sole mPRα was clustered together with mPRα of other fish.The identity was 94％,93％ and 90％ when compared with Southern flounder Paralichthys lethostigma,Atlantic croaker Micropogonias undulates and Japanese medaka Oryzias latipes,respectively.In contrast,identity among the mPRα of tongue sole and that of human Homo sapiens and cattle Bos taurus was low,only 53％.A semi-quantitative RT-PCR was developed to measure mRNA expression levels of mPRα gene of female tongue sole.Tissue expression analysis showed that mPRα mRNA was expressed widely in tongue sole,although the expression level was not homogeneous,mPRα transcripts were highly abundant in brain,kidney,spleen and ovary,less abundant in liver,stomach and muscle.关键词
半滑舌鳎/膜孕激素受体基因/基因克隆/组织表达Key words
Cynoglossus semilaevis Günther /mPRα gene/ Gene cloning/Tissue expression分类
农业科技引用本文复制引用
史宝,李晓晓,柳学周,徐永江,王珊珊,刘芝亮,王妍妍..半滑舌鳎膜孕激素受体基因克隆与组织表达分析[J].渔业科学进展,2013,34(3):61-67,7.基金项目
国家鲆鲽类产业技术体系(CARS-50)、国家自然科学基金(31201982)、公益性农业行业专项项目(200903005)和留学人员科技活动项目择优资助经费共同资助 （CARS-50）
