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异养硝化-好氧反硝化菌株X3的环境适应性

李秋芬 孙雪梅 张艳 刘淮德

渔业科学进展2013,Vol.34Issue(3):120-125,6.
渔业科学进展2013,Vol.34Issue(3):120-125,6.

异养硝化-好氧反硝化菌株X3的环境适应性

Environmental adaptability of heterotrophic nitrifying-aerobic denitrifying bacteria strain X3

李秋芬 1孙雪梅 1张艳 1刘淮德1

作者信息

  • 1. 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所,青岛266071
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摘要

Abstract

For the application of a heterotrophic nitrifying-aerobic denitrifying bacteria strain X3 in mariculture,the effects of several environmental factors,including temperature,salinity,pH,C ∶ N ratio,and carbon source on the growth and nitrogen removing ability of strain X3 were examined in the laboratory conditions,and its sensitivity to different antibiotics was also explored.The results showed that,the optimal condition for strain X3 to grow and remove nitrogen were 28℃,salinity of 30,pH 8.5,using sodium acetate as the carbon source and at a C ∶ N ratio of 10 ∶ 1.This strain grew well and kept over 80% nitrogen removal rate at 25 ~37℃,20~80 salinity,pH 7.5~9.5 with a C ∶ N ratio range of 5 ∶ 1~15.∶ 1 and sodium acetate or glucose as the carbon source.Especially,this strain was able to grow in the medium with salinity of 150.The result of sensitivity to antibiotics indicated that strain X3 was only sensitive to norfloxacin,ofloxacin and polymyxa amphotericin B,while resistant to the other 27 antibiotics.Therefore,it can be concluded that strain X3 has excellent adaptability to the environment of mariculture and huge application potential in mariculture water purification.

关键词

异养硝化-好氧反硝化/盐单胞茵/生物脱氮/环境适应性/海水养殖环境

Key words

Heterotrophic nitrifying-aerobic denitrifying / Halomonas sp./Bio-nitrogen removal / Environmental adaptability/Maricultural environment

分类

生物科学

引用本文复制引用

李秋芬,孙雪梅,张艳,刘淮德..异养硝化-好氧反硝化菌株X3的环境适应性[J].渔业科学进展,2013,34(3):120-125,6.

基金项目

海洋公益性行业科研专项经费项目(200805069)和国家自然科学基金课题(31170113)资助 (200805069)

渔业科学进展

OA北大核心CSCDCSTPCD

2095-9869

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