南京农业大学学报2013，Vol.36Issue(3)：15-21,7.DOI:10.7685/j.issn.1000-2030.2013.03.003
葡萄DELLA家族成员VvRGA和VvRGL1的预测、验证及生物信息学分析
The prediction, validation and bioinformatics analysis of the grape DELLA family members about VvRGA and VvRGL1
摘要
Abstract
In this study,bioinformatics tools were used to make the Arabidopsis DELLA gene sequence as the target sequence to predict 2 DELLA genes(the sequence number are GSVIVP00030538001 and GSVIVP00032990001) in the grape genome sequence,and they were then cloned and sequenced for validation and the bioinformatics analysis was done.After comparing the sequenced result with the related genes of Arabidopsis,they were found they have a higher similarity with Arabidopsis RGA and RGL1:percentage of 63.59％ and 51.28％,and then they were named VvRGA and VvRGL1,respectively.And the bioinformatics analysis was done to know about their amino acid composition,physicochemical properties and the secondary and tertiary structure of protein.The results showed that they both have the conserved GRAS domain and the DELLA domain ; they have the different number of amino acids,amino acid sequence of hydrophobic with Arabidopsis; their secondary structure is mainly α-helix and random coil,the percentages of α-helix are 47.98％ and 50.52％,the percentages of random coil are 38.77％ and 35.28％ ;their tertiary structures are very similar,and only the number of α-helix and β-sheet is different.This may make their function differently.关键词
葡萄/VvRGA/VvRGL1/基因预测/验证/分析Key words
grape / VvRGA / VvRGL1 / gene prediction / verification/ analysis分类
农业科技引用本文复制引用
任国慧,上官凌飞,房经贵,慕茜,冷翔鹏,孙欣..葡萄DELLA家族成员VvRGA和VvRGL1的预测、验证及生物信息学分析[J].南京农业大学学报,2013,36(3):15-21,7.基金项目
江苏省现代园艺科学优势学科建设工程专项 （）
