南京农业大学学报2013，Vol.36Issue(3)：111-116,6.DOI:10.7685/j.issn.1000-2030.2013.03.019
植物多酚对模拟胃酸体系中亚硝酸盐清除能力与亚硝胺生成的影响
Effects of polyphenols on the nitrite scavenging and N-nitrosamine formation in simulated gastric acid
摘要
Abstract
In simulated gastric acid,effects of tea polyphenols (TP) and grape seed polyphenols (GP) on nitrite scavenging and nitrosamine formation were determined,and their antioxidant activities were assessed.The c.ontent of N-nitrosodiethylamine(NDEA) and nitrite were determined by high performance liquid chromatography (HPLC) and spectrophotometric method,respectively.The results showed that with plant polyphenols contents increasing and reaction times extending,the nitrite scavenging effect of TP and GP increased.The scavenging effect of TP was better than that of GP.With the increase of TP and GP contents,the relative content of NDEA increased gradually.When the contents of TP and GP were 0.5 and 1.0 mg· mL-1,the relative content of NDEA reached the maximum.Afterwards,the content of NDEA declined and finally the NDEA formation was inhibited completely.Antioxidant activity analysis showed that with the increase of polyphenols contents,the antioxidant capacity increased gradually.Also,TP exhibited the higher antioxidant activity than GP.These results confirmed that at least in our experimental conditions,TP and GP had high nitrite scavenging and antioxidant ability,and possessed a dual role of facilitation and inhibition on NDEA formation.关键词
茶多酚/葡萄籽提取物/亚硝酸盐/亚硝胺/抗氧化性Key words
tea polyphenols / grape seed polyphenols / nitrite / nitrosamine / antioxidant分类
轻工纺织引用本文复制引用
李玲,张汆,周光宏,徐幸莲..植物多酚对模拟胃酸体系中亚硝酸盐清除能力与亚硝胺生成的影响[J].南京农业大学学报,2013,36(3):111-116,6.基金项目
教育部高校博士点基金项目(20090097110002) （20090097110002）
国家公益性行业(农业)科研专项(200903012) （农业）
