生物加工过程2013，Vol.11Issue(3)：1-6,6.DOI:10.3969/j.issn.1672-3678.2013.03.001
利用甘蔗糖蜜发酵生产花生四烯酸的研究
Arachidonic acid production from cane molasses by Mortierella alpina
摘要
Abstract
Arachidonic acid (ARA) fermentation by Mortierella alpina was carried out using cane molasses.Several different preparation methods were investigated to evaluate the optimal process of cane molasses method.The results indicated that sulfuric acid process was the best method.The cultivation parameters were as follows:reducing sugar concentration 80 g/L,nitrogen resource concentration 6 g/L,inoculum density 20％,initial pH 6.0,and cultivation temperature 25 ℃.The resultant dry cell weight,total lipid content,arachidonic acid yield,and sugar utilization were 28.5 g/L,11.7 g/L,3.68 g/L,and 94.5％,respectively.关键词
花生四烯酸/糖蜜/发酵/高山被孢霉Key words
arachidonic acid / cane molasses / fermentation / Mortierella alpina分类
生物科学引用本文复制引用
彭超,黄和,纪晓俊,刘欣,聂志奎,邓中涛..利用甘蔗糖蜜发酵生产花生四烯酸的研究[J].生物加工过程,2013,11(3):1-6,6.基金项目
江苏省六大人才高峰项目(2008-D-63) （2008-D-63）
江苏省研究生创新计划(CX10B_175Z) （CX10B_175Z）
