生物加工过程2013，Vol.11Issue(3)：18-23,6.DOI:10.3969/j.issn.1672-3678.2013.03.004
诱导子对藏红花悬浮培养细胞生产藏红花色素的影响
Effects of elicitors on saffron pigment production in cell suspension cultures of Crocus sativus L.
摘要
Abstract
The effects of chitosan,chitosan oligosaccharides (COS),methyl jasmonate (MJ),salicylic acid (SA),and copper ions(Cu2+) on cell growth and saffron pigment production in cell suspension cultures of C.sativus were investigated.The results showed that COS(1-500 mg/L),chitosan(≤ 10 mg/L),M J(≤ 10μmol/L),SA(≤10 μmol/L),and Cu2+ (≤1 μmol/L) had no significant effects on saffron cell growth,whereas chitosan(≥100 mg/L),MJ(≥100 μmol/L),SA(≥ 100 μmol/L),and Cu2+ (≥ 10 μmol/L) at high concentrations obviously inhibited the cell growth.The effects of elicitors were significant on saffron pigment biosynthesis,and had relations with the concentrations of elicitors and the timing of elicitation.MJ was the best elicitor,the content of saffron pigment reached 28.57 mg/g DW by adding 100 μmol/L MJ on day 0,and it was higher by 177.9％ than the control.Cu2+ was the second,the content of saffron pigment reached 19.82 mg/g DW by adding 500 μmol/L Cu2 + on day 4,and it was higher by 108.2％ than the control.Chitosan and COS were the third,the contents of saffron pigment reached 18.33 mg/gDW and 17.39 mg/gDW by adding 100 mg/L chitosan and oligochitosan on day 14,and it was higher by 69.1％ and 69.0％ than the control,respectively.The last one is SA,the content of saffron pigment reached 14.65mg/gDW by adding 10 μmol/L SA on day 14 and it was higher by 45.4％ than the control.关键词
细胞悬浮培养/藏红花/诱导子/藏红花色素Key words
cell suspension culture / Crocus sativus L./ elicitor/ saffron pigment分类
农业科技引用本文复制引用
孙镇,袁丽红,吴频梅..诱导子对藏红花悬浮培养细胞生产藏红花色素的影响[J].生物加工过程,2013,11(3):18-23,6.基金项目
国家重点基础研究发展计划(973计划)(2009CB724700) （973计划）
