生物加工过程2013，Vol.11Issue(3)：29-33,5.DOI:10.3969/j.issn.1672-3678.2013.03.006
利用双水相萃取技术分离纯化尿激酶
Separation and purification of urokinase by aqueous two-phase extraction
摘要
Abstract
An aqueous two-phase extraction system using ethanol/(NH4)2 SO4 to separate urokinase was established.Factors of ethanol/(NH4)2SO4 ratio,pH,inorganic salt and crude enzyme concentration influencing the purification were investigated.The results showed that 65％ cold ethanol,10％ (NH4)2SO4,pH8.0,crude enzyme 30％.Under the optimal conditions,no inorganic salt was added.Purification urokinase was up to 9 times,and the recovery was 92％.关键词
双水相/尿激酶/萃取/纯化Key words
aqueous two-phase / urokinase / extraction / purification分类
化学化工引用本文复制引用
李兵,朱宝成..利用双水相萃取技术分离纯化尿激酶[J].生物加工过程,2013,11(3):29-33,5.基金项目
邢台市科技支撑计划(2012ZC044) （2012ZC044）
