西北林学院学报2013，Vol.28Issue(4)：66-70,5.DOI:10.3969/j.issn.1001-7461.2013.04.13
栓皮栎不同变异类型RAPD分析
RAPD Analysis on Different Variant Types of Quercus variabilis
摘要
Abstract
In order to speed up the genetic improvement of Quercus variabilis,genomic DNAs of 24 materials of four types collected from the northern slope of Qinling Mountains were amplified by RAPD (random amplified polymorphic DNA) technology with 35 primers screened from 235 arbitrary primers.The total genetic diversity level of natural Q.variabilis population was high,the genetic differentiation of natural Q.variabilis population was great and its genetic variation was high.The natural population of Q.variabilis was divided into four variant types:thick-bark-deep-crack type,thick-bark-shallow-crack type,thin-barkshallow-crack type,and thin-bark-deep-crack type.The genetic basis of the division was demonstrated by UPGMA cluster analysis,which coincided with its morphological taxonomy basically.Polymorphic loci,percentage of polymorphic loci,average observed number of alleles,Shannon information index and Nei's genetic diversity indicated that the changes of genetic diversity of all types were consistent:thick-barkdeep-crack type ＞ thin-bark-shallow-crack type ＞ thin-bark-deep-crack type ＞ thick-bark-shallow-crack type,and their genetic diversity was high.Genetic germplasm resources were extremely abundant and genetic improvement potentials were great in Q.variabilis.关键词
栓皮栎/变异类型/RAPD/遗传多样性Key words
Quercus variabilis/ variant type/ RAPD/ genetic diversity分类
农业科技引用本文复制引用
任耀忠,张文辉,周建云..栓皮栎不同变异类型RAPD分析[J].西北林学院学报,2013,28(4):66-70,5.基金项目
国家林业局林业公益性行业专项(201004011) （201004011）
国家自然科学基金项目(30872018) （30872018）
陕西省重点基础研究项目(2009JZ005). （2009JZ005）
