中国临床药理学与治疗学2013，Vol.18Issue(5)：495-500,6.
七氟烷、异氟烷对幼鼠脑细胞凋亡和远期学习记忆功能的影响
Effects of sevoflurane and isoflurane on neuroapoptosis and the long-term learning ability in newborn rat brain
摘要
Abstract
AIM:To investigate the changes of neuroapoptosis in brain and learning ability after neonatal mice exposed to inhaled sevoflurane or isoflurane.METHODS:Ninety postnatal day(P)7 Wistar rats were randomly divided into 5 groups:group A sham anesthesia,group B 3.6％ sevoflurane for 2 h,group C 3.6％sevoflurane for 6 h,group D 2.3％ isoflurane for 2 h and group E 2.3％ isoflurane for 6 h.Animals from each group were perfused transcardially with 0.1 mol phosphate buffer containing 4％ paraformaldehyde 6 h after the end of anesthesia,and then the brains were exposed for immunohisochemistry,caspase-3 positive cells were detected.Behavioral studies with Morris water maze test were performed separately when the rats were 5-week-old and 14-week-old.RESULTS:The amounts of caspase-3 positive cells in the rats brain of Group B,C,D and E were greater than that in Group A,and the amount in group C was greater than that in gourp B,in group E more than that in group B(P＜0.05).When face the spatial reference memory task or space exploration task,rats from different groups make it uniformly.Anesthetic rats and control rats performed similarly in terms of path length when learning to swim to the platform during locating trials.No rats showed significantly higher levels of retention during the probe trials in terms of target quadrant time,platform crossings and platform location time(P＞0.05),but the number of platform crossings in group B,C,D and E was significantly higher than that in gourp A(P＜0.05).CONCLUSION:Exposure to the concentration of 3.6％ sevoflurane or 2.3 ％ isoflurane could cause brain cell apoptosis of newborn rats.Isoflurane could cause more insults to brain than sevoflurane at an equivalent concentration.The memory ability to pessimal stimulation is decreased as the anesthesia mice 5weeks old,such changes recede along with the growth of rats.Exposure to sevoflurane and isoflurane does not affect the spatial reference memory of newborn rat during their growth.关键词
七氟烷/异氟烷/幼鼠/caspase-3/空间记忆Key words
Sevoflurane/ Isoflurane/ Newborn rats/ caspase-3/ Spatial reference memory分类
医药卫生引用本文复制引用
斯小龙,李国政,刘小南,徐宏明,彭从斌..七氟烷、异氟烷对幼鼠脑细胞凋亡和远期学习记忆功能的影响[J].中国临床药理学与治疗学,2013,18(5):495-500,6.基金项目
国家自然科学基金(81271204) （81271204）
浙江省自然科学基金(Y205219) （Y205219）
