中国农业科学2013,Vol.46Issue(3):623-629,7.DOI:10.3864/j.issn.0578-1752.2013.03.019
中国矮马群体mtDNA Cytb基因遗传多样性及系统进化研究
Genetic Diversity and Phylogenetic Relationship Among Five Pony Populations of China
摘要
Abstract
[Objective] In order to provide date and scientific basis for utilization and protection ofbiodiversity,diversity and phylogenetic relationships of five pony populations,136 samples were studied.[Method] The PCR amplification and sequencing of mtDNA Cytb gene,the date analyzsis ways of biologic informatics were used.[Result] A total of 27 haplotypes were detected,and 40 polymorphic loci which account for 3.51% of the analyzed sites were found.The Yunnan and Guizhou populations had the highest haplotype diversity (Hd =0.869) and nucleotide diversity (Pi =0.0435),respectively,whereas the Yuexi and Ningqiang populations had the lowest respectively,but not far from each population.Each branch of the phylogenetic tree and NETwork results all had the information of five pony populations.[Conclusion] The five pony populations had higher genetic diversity in all,the Yunnan,Guizhou and Debao ponies had relatively higher genetic diversity,but Yuexi and Nongqiang pony were low.the phylogenetic research suggested that extensive gene flow exists among the five pony populations,and this study has provided evidence for Chinese ponies having multiple maternal origins.关键词
中国矮马/Cytb/遗传多样性/系统进化Key words
Chinese pony/ Cytb/ genetic diversity/ phylogenetic relationship引用本文复制引用
徐龙鑫,杨胜林,李爱萍,乜玉丽,杨海兵,戴焰..中国矮马群体mtDNA Cytb基因遗传多样性及系统进化研究[J].中国农业科学,2013,46(3):623-629,7.基金项目
国家自然科学基金(30960162) (30960162)