浙江农林大学学报2013，Vol.30Issue(3)：401-405,5.
日本蟾蜍皮肤胸腺素α原cDNA的克隆及序列分析
Cloning and sequence analysis of prothymosin-α cDNA of Bufo japonicus formosus
摘要
Abstract
To study the polypeptide effective components included in the skin of Bufo japonicus formosus (Japanese toad) and their secretions,the full length cDNAs were screened from the plasmid cDNA library of adult Japanese toad skin using colony polymerase chain reaction with a pair of primers,SP6 (the upstream primer of the vector) and poly(T) (a self-designed primer recognizing the area connecting polyA tail of cDNA and the vector).Results showed that a prothymosin-α (ProTα) cDNA was obtained from l 344 colonies checked,whose transcript was 1 480 bp in length consisting of 125 bp in the 5 prime untranslated region (5' UTR) and 1 016 bp 3' UTR with a complete open reading frame (ORF) of 339 bp encoding a polypeptide of 112 amino acid residues.The homologous analysis indicated a similarity between B.japonicus formosus and Rana esculenta of up to 82％,but for other species it was 54％-73％.Previous studies have shown significant anti-tumor effects of ProTα; therefore,this study could contribute to further studies on ProTα.关键词
动物学/日本蟾蜍/ProTα/基因克隆/序列分析Key words
zoology/Bufo japonicus formosus/ProTα/gene cloning/ sequence analysis分类
农业科技引用本文复制引用
宋敏国,袁进强,杨仙玉,张姝芳,诸葛慧,徐跃..日本蟾蜍皮肤胸腺素α原cDNA的克隆及序列分析[J].浙江农林大学学报,2013,30(3):401-405,5.基金项目
国家自然科学基金资助项目(31071181) （31071181）
浙江省科技创新活动计划(新苗人才计划)项目(2011R412042,2010R412003) （新苗人才计划）
