作物学报2013，Vol.39Issue(4)：609-616,8.DOI:10.3724/SP.J.1006.2013.00609
大豆粒形性状QTL的精细定位
Fine Mapping of Quantitative Trait Loci for Seed Shape Traits in Soybean
摘要
Abstract
Based on the target interval region of SSR markers Satt331-Satt592,we constructed 356 RHL-F2 individuals (population Ⅰ) and 168 recombinant families (population Ⅱ) derived from 504 F2∶6 families of the direct and reciprocal crosses of Lishuizhongzihuang with Nannong 493-1 to fine map quantitative traits loci (QTLs) for seed length (SL),width (SW),and length-to-width (SLW) ratio in soybean using composite interval mapping (CIM) and lasso approaches.Among the 168 recombinant families,only one recombinant individual was selected from a same F2∶6 family so that 142 recombinant families were constructed as population Ⅲ.Response variable for QTL analysis was as original observation,and the value corrected by the associated marker information.As a result,markers associated with SL were O19 and S21/Sat t331 from lasso method and S21-S22 and O23-O19 from CIM method; marker associated with SW was O19/O21 from lasso method and O23-O19/O19-O21 from CIM method; and the QTL associated with markers S21-S22 for SLW was derived from QTL for SL,and the QTL with markers O23-019/O19-021 for SLW was derived from QTLs for SL and SW.This indicates that QTL associated with markers Satt331-Satt592 in our study was further partitioned into two pleiotropic QTLs,which were associated with markers S21-S22 and markers O23-O19/O19-O21.According to the annotation project database,Glyma10g35240 and Glyma10g34980 might be candidate genes for seed shape traits.关键词
大豆/粒形/数量性状基因座/精细定位/候选基因Key words
Soybean/ Seed shape trait/ Quantitative trait locus/ Fine mapping/ Candidate gene引用本文复制引用
牛远,谢芳腾,布素红,谢尚潜,韩世凤,耿青春,刘兵,章元明..大豆粒形性状QTL的精细定位[J].作物学报,2013,39(4):609-616,8.基金项目
本研究由教育部新世纪优秀人才支持计划项目(NECT-05-0489),江苏省自然科学基金项目(BK2008335)和中央高校基本科研业务费专项资金创新团队项目(KYT201002)资助. （NECT-05-0489）
