作物学报2013，Vol.39Issue(4)：617-625,9.DOI:10.3724/SP.J.1006.2013.00617
基于染色体单片段代换系的水稻粒形QTL定位
Mapping of QTLs for Grain Shape Using Chromosome Single Segment Substitution Lines in Rice (Oryza sativa L.)
摘要
Abstract
Grain shape is one of the important factors determining rice yield and grain quality,it is a typical quantitative trait controlled by multiple genes.As novel research material,chromosome single segment substitution lines are useful in QTL identification because of minimizing the interference of genetic background among plants.In this study,QTLs for grain shape were identified with 119 rice chromosome single segment substitution lines derived from Nipponbare as donor parent in the background of Guanglu'ai 4 by one-way analysis of variance and Dunnett's test for means comparisons between chromosome single segment substitution lines and the recipient parent Guanglu'ai 4.A total of 39 QTLs for grain shape were identified on 11 chromosomes except chromosome 10 with a significance of P≤0.001.Among them,19 QTLs were identified for grain length,with the additive effect from 0.18 mm to 1.06 mm and the additive effect percentages from 2.40％ to 14.13％; 14 QTLs were identified for grain width,with the additive effect from 0.09 mm to 0.31 mm and the additive effect percentages from 2.71％ to 9.15％; and 6 QTLs were identified for grain thickness,with the additive effect from 0.05 mm to 0.10 mm and the additive effect percentages from 2.14％ to 4.46％.The results are important for the QTLs cloning and molecular breeding ofrice grain shape.关键词
水稻/单片段代换系/数量性状/粒形/代换作图Key words
Rice/ Chromosome single segment substitution lines/ Quantitative trait loci/ Grain shape/ Substitution mapping引用本文复制引用
王军,朱金燕,周勇,杨杰,范方军,李文奇,梁国华,仲维功..基于染色体单片段代换系的水稻粒形QTL定位[J].作物学报,2013,39(4):617-625,9.基金项目
本研究由国家自然科学基金项目(31101131),国家科技支撑计划重大项目(2011BAD16B03),江苏省自然科学基金项目(BK2011666),高等学校博士学科点专项科研基金项目(20103250110004),江苏省科技支撑计划项目(BE2012309)和江苏省农业自主创新资金[CX(12)5017]资助. （31101131）
