作物学报2013，Vol.39Issue(4)：626-631,6.DOI:10.3724/SP.J.1006.2013.00626
水稻矮秆脆性突变体dbc1的鉴定与基因定位
Identification and Gene Mapping of Dwarf and Brittle Culm Mutant dbc1 in Oryza sativa
摘要
Abstract
One dwarf and brittle culm mutant dbc1 was identified from the progeny of restorer line Jinhuil0 which seeds were treated by ethyl methanesulfonate (EMS).From the seedling stage to the mature stage,dbc1 showed obvious characteristics of dwarfism and frangibility.In detail,the mutant shortened each intemode with a plant height of 58.93 cm,belonging to the dn type of dwarfism mutation.Treated by GA3,the dbc1 showed more insensitive compared with its original parent Jinhuil0.One thousand grain weight,seed-setting rate,and tiller numbers did not change while panicle length,grain number per panicle and full grain number decreased significantly in the mutant.Further analysis showed that mechanical strengths declined significantly in the mutational leaf blades and culms.Interestingly,cellulose content was not changed and lignin content slightly decreased compared with those of wild type,with a significant difference at P＜0.05 between them.Genetic analysis indicated that the mutation was controlled by one recessive nuclear gene.In this paper,DBC1 gene was finally mapped between SSR markers RM13943 and RM13952 on chromosome 2 based on 886 F2 mutational plants of Xinong1A/dbc1.In the restricted region,the physical distance was 197 kb and contained 52 annotated genes.These results provide a foundation for DBC1 gene cloning and its application in the molecular breeding.关键词
水稻/矮秆/脆性/赤霉素钝感/基因定位Key words
Rice (Oryza sativa)/ Dwarf/ Brittle culms/ Gibberellins-insensitive/ Gene mapping引用本文复制引用
桑贤春,杜川,王晓雯,杨正林,凌英华,赵芳明,李云峰,何光华..水稻矮秆脆性突变体dbc1的鉴定与基因定位[J].作物学报,2013,39(4):626-631,6.基金项目
本研究由国家转基因生物新品种培育科技重大专项(2011ZX08001-002)和重庆市科技攻关计划项目(CSTC2012GGc80002)资助. （2011ZX08001-002）
