动物营养学报2013，Vol.25Issue(7)：1519-1525,7.DOI:10.3969/j.issn.1006-267x.2013.07.016
乙酸浓度对奶牛乳腺上皮细胞甘油三酯含量及瘦素和过氧化物酶增殖物激活受体y基因表达量的影响
Effects of Acetate Concentration on Triacylglycerol Content and Expression Levels of Leptin and PPARγ Genes in Bovine Mammary Epithelial Cells
摘要
Abstract
This study was conducted to determine the effects of acetate concentration on triacylglycerol (TAG) content,as well as expression levels of leptin and peroxisome proliferator-activated receptor-γ (PPARγ) genes in bovine mammary epithelial cells (BMECs).The 3th passage BMECs with proper densities were cultured for 48 h,then cells were divided into six treatments.Culture mediums containing different concentrations of acetate (0,4,6,8,10 and 12mmol/L) were used in each treatment,respectively.Cells were continuous incubated in an incubator with 5％ CO2 at 37 ℃ for 48 h,then were collected for the analysis of lipid droplet formation,TAG content,and expression levels of leptin and PPARγ genes.The results showed as follows:with the increase of acetate concentration,lipid droplets was increased in BMECs; TAG content and PPARy gene expression level were significantly increased linearly or quadratically (P ＜ 0.05),and 10 and 12 mmol/L were optimal concentrations; leptin gene expression level was up-regulated by acetate at a certain concentration,and 8 and 10 mmol/L were optimal concentrations.In conclusion,acetate can stimulate lipid droplet formation,TAG accumulation and expression levels of leptin and PPARγ genes in BMECs; in the present study,10 to 12 mmol/L acetate has better effects on PPARγ gene expression,and 8 to 10 mmol/L acetate has better effects on leptin gene expression.关键词
乙酸/奶牛乳腺上皮细胞/瘦素/过氧化物酶增殖物激活受体γKey words
acetate/ BMECs / leptin / PPARγ分类
农业科技引用本文复制引用
齐利枝,生冉,闫素梅,赵艳丽..乙酸浓度对奶牛乳腺上皮细胞甘油三酯含量及瘦素和过氧化物酶增殖物激活受体y基因表达量的影响[J].动物营养学报,2013,25(7):1519-1525,7.基金项目
奶业"973计划"(2011CB100800) （2011CB100800）
