昆明医科大学学报2013，Vol.34Issue(4)：32-34,42,4.
高同型半胱氨酸血症骨骼肌TRB3表达与糖代谢的关系
The Relationship between Expression of TRB3 and Glycometabolism in Skeletal Muscle of Hyperhomocysteinemia
摘要
Abstract
Objective To study expression of tribbles 3 (TRB3) in the skeletal muscle tissue in herpyhomocysteinemia animal model,and explore the relationship between TRB3 and glycometabolism.Methods Twenty mice,six weeks of age and healthy,were randomly divided into the control group (n =10) and the hyperhomocysteinemia group (n =10) Normal food was fed in the control group; Normal food and 1.5％ (mass fraction) methionine were fed in the hyperhomocysteinemia group.After mice in the hyperhomocysteinemia group and the control group were fed for three months,the levels of blood glucose were determined in the feeding.The structure of skeletal muscle cells was observed by HE staining.The levels of TRB3,Akt and p-Akt (Ser-473) were observed with Western blot.Results The feeding blood glucose levels in the hyperhomocysteinemia group were higher than in the control group (P ＜ 0.05).The structure of skeletal muscle cells was not obvious anomaly by HE staining.The levels of TRB3 protein in the hyperhomocysteinemia group were increased compared with the control group (P ＜0.05),however,the expressions of the p-Akt (Ser-473) protein were decreased in the herpyhomocysteinemia group compared with the control group.The levels of Akt were not different in the herpyhomocysteinemia group compared with the control group (P ＞ 0.05).Conclusion Homocysteine may induce expression of TRB3 in skeletal muscle tissue,resulting in inhibiting PI3-K/Akt signal pathway and leading to disturbance of glycometabolism.关键词
高同型半胱氨酸血症/骨骼肌组织/糖代谢/TRB3Key words
Hyperhomocysteinemia/ Skeletal muscle tissue / Glycometabolism/ Tribbles 3分类
医药卫生引用本文复制引用
凌大庆,郭家智,李树德,顾玉,李若楠..高同型半胱氨酸血症骨骼肌TRB3表达与糖代谢的关系[J].昆明医科大学学报,2013,34(4):32-34,42,4.基金项目
NSFC-云南联合基金资助项目(U1132606) （U1132606）
云南省科技厅-昆明医科大学联合专项基金资助项目(2009CD213) （2009CD213）
云南省应用基础研究面上金资助项目(2010CD075,2011CD223) （2010CD075,2011CD223）
