昆明医科大学学报2013，Vol.34Issue(4)：103-104,2.
噬菌体生物扩增技术在结核分枝杆菌利福平耐药性检测中的应用
Application of the Phage Amplifted Biologically Assay in Drug Resistance Testing of Rifampcin of Mycobacterium Tuberculosis
李晓非 1梁桂亮 1汪亚玲 1苏俊华 1李利国1
作者信息
- 1. 昆明市第三人民医院,云南昆明 650041
- 折叠
摘要
Abstract
Objective To evaluate the clinic application value of Phage amplified biologically assay (PhaB) in drug resistance testing of Rifampcin of Mycobacterium tuberculosis.Method The sputum samples of Mycobacterium tubercuolosis in 78 cases was detected by PhaB method about the drug resistance of Rifampcin and compared with the results of concentration ratio (CR) method's.Result CR method as a standard of criterion,the sensitivity,specitivity and accuracy of PhaB method in testing Rifampcin resistance was 84.0％,94.33％ and 91.03％,respectively.Conclusion Compared with CR methods,PhaB method has higher sensitivity and specitivity,so it should be as a better choice for detecting drug resistance of Mycobacterium tuberculosis as it is not only simple but also rapid and without requiring sophisticated facilities.关键词
噬菌体扩增法/比例法/利福平/灵敏度Key words
PhaB/ Concentration ratio method/ Rifampicin / Sensitivity分类
医药卫生引用本文复制引用
李晓非,梁桂亮,汪亚玲,苏俊华,李利国..噬菌体生物扩增技术在结核分枝杆菌利福平耐药性检测中的应用[J].昆明医科大学学报,2013,34(4):103-104,2.
