南京医科大学学报（自然科学版）2013，Vol.33Issue(5)：569-574,6.DOI:10.7655/NYDXBNS20130502
慢病毒介导ATP7B基因转染对骨髓间充质干细胞在高铜环境中生存能力的影响
The protective effect of overexpressed ATP7B in bone marrow-derived mesenchymal stem cells against copper toxicity
摘要
Abstract
Objective:To investigate the role of ATP7B overexpression for the survival of bone marrow-derived mesenchymal stem cells (MSCs) of LEC rat in presence of various copper concentrations in vitro.Methods:Bone marrow cells were isolated from the femurs of LEC rat by flushing with rat MSCs growth medium;the lentivirus package system carrying ATP7B/green fluorescent protein (GFP) gene was constructed,and MSCs were infected by the virus.The expression of ATP7B was measured by immunofluorescence,Western blot and Real-time PCR.The SRB assay was used to analyze the viability of MSCs and HepG2 in the presence of various copper concentrations.Results:We successfully built up LEC rat MSCsATP7B which can stably express high level of ATP7B in vitro.In the SRB assay,the viability of MSCsATP7B was significantly higher than that of either MSCsGFP or HepG2 in the presence of high copper concentrations.Conclusion:MSCs of LEC rat rescued by ATP7B can effectively antagonize copper toxicity in vitro,and may represent a novel strategy for therapy of Wilson disease.关键词
ATP7B/骨髓间充质干细胞/Wilson病/LEC大鼠Key words
ATP7B/bone marrow-derived mesenchymal stem cells/ Wilson disease/ LEC rat分类
生物科学引用本文复制引用
邵存华,陈圣林,董天赋,成峰..慢病毒介导ATP7B基因转染对骨髓间充质干细胞在高铜环境中生存能力的影响[J].南京医科大学学报（自然科学版）,2013,33(5):569-574,6.基金项目
国家自然基金面上项目(81070324) （81070324）
省卫生厅重点项目(H201102) （H201102）
卫生厅开放课题(ZX05200906) （ZX05200906）
省六大人才高峰项目(2009) （2009）
