农业环境科学学报2013，Vol.32Issue(4)：689-696,8.DOI:10.11654/jaes.2013.04.005
高效氯氰菊酯对克氏原螯虾(Procambarus clarkii)抗氧化酶活性的影响
Effects of Beta-cypermethrin on Antioxidant Enzymes Activities of Procambarus clarkii
摘要
Abstract
Beta-cypermethrin,a type Ⅱ pyrethroid insecticide,is widely used in agriculture around the world.Although much research on the toxicity of beta-cypermethrin has been done in fishes,little is so far available regarding its toxicity to crustaceans,such as shrimp,crab and crayfish.To investigate the acute toxic effect of beta-cypermethrin on Procambarus clarkii,the crayfishes were exposed to various concentrations of beta-cypermethrin(0 μg· L-1,0.005 μg· L-1,0.01 μg· L-1,0.02 μg· L-1 and 0.04 μg· L-1) until 96 h according to the 96 h LC50 values (0.2 μg· L-1) measured previously.Activities of antioxidant enzymes viz.superoxide dismutase (SOD) and catalase (CAT),and contents of malondialdehyde(MDA) in gill,hepatopancreas and serum were determined at 24 h,48 h,72 h and 96 h,respectively.Results indicated that SOD activities first increased then declined in different tissues during the 96 h exposure,and that CAT activities showed the similar trends.MDA contents in all treatments were higher than those in the control group at all the time and they increased with the exposed time and concentration increasing.After 96 h,the activities of SOD in hepatopancreas,gill and serum at 0.04 μg· L-1 treatment decreased by 28.3％,44.1％ and 27.2％,compared with control group,respectively.Similarly,the activities of CAT in different tissues decreased by 38.1％,39.4％ and 12.3％,respectively.MDA contents in hepatopancreas,gill and serum were 0.72 times,1.09 times and 00.46 times higher than those in the control group,respectively.This study showed that beta-cypermethrin was able to induce oxidative stress and had significant negative effects on antioxidant enzyme activities of Procambarus clarkii.关键词
高效氯氰菊酯/克氏原螯虾/超氧化物歧化酶(SOD)/过氧化氢酶(CAT)/丙二醛(MDA)Key words
beta-cypermethrin/ Procambarus clarkii/ superoxide dismutase(SOD)/ catalase(CAT)/ malondialdehyde (MDA)分类
资源环境引用本文复制引用
毛阿敏,魏克强,赵辉,刘婉莎,刘娥娥,吕虹瑞..高效氯氰菊酯对克氏原螯虾(Procambarus clarkii)抗氧化酶活性的影响[J].农业环境科学学报,2013,32(4):689-696,8.基金项目
教育部高等学校博士学科点专项科研基金(200801081012) （200801081012）
山西省人才引进与开发专项(2008) （2008）
