农业环境科学学报2013，Vol.32Issue(4)：805-810,6.DOI:10.11654/jaes.2013.04.022
一株异养硝化细菌的分离鉴定和脱氮特性研究
Isolation and Denitrification Characteristics of a Heterotrophic Nitrification Bacterium
摘要
Abstract
The present paper dealt with isolation and denitrification characteristics of a heterotrophic nitrification bacterium,which isolated from the high NH3-N swine wastewater.Based on the screening methods published by some scientists,a isolating method in the study was put forward to used an acetamide as a sole carbon and nitrogen source to screen a heterotrophic nitrifying bacterium from the swine wastewater.An efficient heterotrophic nitrification bacterium was isolated and identified with 16S rDNA sequence.The phylogenetic analysis of the strain was performed based on the 16S rDNA sequence.The efficiency of heterotrophic nitrification was detected for the strain.The strain FJAT-14899 was identified to be Paracoccus denitrificans with the aid of 16S rDNA sequencing.The removal rates for the total concentrations of nitrogen and NH4-N were 81.7％ and 53.7％ in 12 h cultural time.The final removal rates of the total nitrogen and NH4+-N were 90.1％ and 61.3％ after 24 h culturing without accumulations of nitrite nitrogen and nitrate nitrogen.Paracoccus denitrificans FJAT-14899 had a higher removal rate of NH4+-N with promissing application in water treatment.关键词
异养硝化细菌/分离/脱氮副球菌/脱氮能力Key words
heterotrophic nitrification bacterium/ isolation/ Paracoccus denitrificans./ denitrification capacity分类
资源环境引用本文复制引用
王洁,蓝江林,刘波..一株异养硝化细菌的分离鉴定和脱氮特性研究[J].农业环境科学学报,2013,32(4):805-810,6.基金项目
国家科技支撑计划:东南地区农牧废弃物多级循环利用技术集成与示范(2012BAD14B15) （2012BAD14B15）
国际合作专项:规模化生猪养殖污染微生物治理关键技术的联合研发(2012DFA31120) （2012DFA31120）
