青岛大学医学院学报2013，Vol.49Issue(4)：312-314,3.DOI:10.11712/qdyxy201304010
HO-1对帕金森病模型小鼠多巴胺能神经元的作用
EFFECTS OF HO-1 ON DOPAMINERGIC NEURONS IN MODEL MOUSE OF PARKINSON DISEASE
摘要
Abstract
Objective To investigate the effect of inhibition of heme oxgenase-1 (HO-1) on dopaminergic neurons in the substantia nigra (SN) in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of Parkionson disease.Methods C57BL/6 mice were divided into three groups (control group,MPTP group and ZnPPIX+MPTP group) in random,with six in each group.A mouse model of Parkinson's disease was created by i.p.injection of MPTP (30 mg/kg.),once daily for five days.The mice in the MPTP group were given normal saline (2 μL,i.c.v.) 4 h before MPTP injection-for eight days; those in ZnPPIX+MPTP group,microinjection of ZnPPIX (50 μg/kg) was given,4 h before the injection of MPTP,for eight days; for those in the control,MPTP and ZnPPIX were replaced by normal saline.The expression of HO-1 and TH in the SN was assessed by western blots.Results The expression of HO-1 in mouse SN of ZnPPIX+MPTP was lower than that of the control group and MPTP group (F=6.844 ;q =5.943,4.444 ;P＜0.05).In MPTP group and ZnPPIX+MPTP group,the TH expression in SN area was lower than that in the control (F=5.095 ;q=4.303,4.030;P＜0.05),the difference of TH expression between ZnPPIX+MPTP and MPTP group was not significant (q=0.234,P＞0.05).Conclusion Inhibiting the expression of HO-1 does not play a significant role in deteriorating the degeneration of dopaminergic neurons of Parkinson's mouse induced by MPTP.关键词
帕金森病/血红素加氧酶-1/1-甲基-4-苯基-1,2,3,6-四氢吡啶/多巴胺能神经元Key words
Parkinson disease/ heme oxygenase-1/ 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine/ dopaminergic neuron分类
生物科学引用本文复制引用
徐效凤,宋宁,姜宏,谢俊霞..HO-1对帕金森病模型小鼠多巴胺能神经元的作用[J].青岛大学医学院学报,2013,49(4):312-314,3.基金项目
山东省自然科学基金重点项目(ZR-2012HZ005) （ZR-2012HZ005）
