石油化工2013，Vol.42Issue(6)：604-609,6.
反胶束体系中脂肪酶催化乙酸甲酯与棉籽油合成生物柴油
Synthesizing Biodiesel from Cottonseed Oil and Methyl Acetate with Lipase Catalyst in Reverse Micelle System
摘要
Abstract
The synthesis of biodiesel from cottonseed oil and methyl acetate catalyzed by free lipase in bis (2-ethylhexyl) sulfosuccinate(AOT) reverse micellar systems was studied.The effects of the concentration of AOT,mass ratio of water to AOT(ξ0),lipase dosage,buffer pH,n(methyl acetate):n(cottonseed oil),reaction temperature and rotating speed on the synthesis were investigated.Single factor experiments and orthogonal experiments were carried out to optimize the reaction conditions.The yield of fatty acid methyl ester can reach 65.53％ under the optimal conditions of ξ0 7,AOT concentration 40 mmol/L,lipase dosage 10 mg(solution volume 2.5 mL),buffer pH 7,n(dimethyl carbonate):n(cottonseed oil) 3,reaction temperature 35 ℃ and rotating speed 160 r/min.The effects of the conditions arrange in order of buffer pH＞reaction temperature＞n(methyl acetate):n(cottonseed oil)＞lipase dosage＞ AOT concentration ＞rotating speed＞ξ0.The stability of the lipase with methyl acetate as a receptor is better than those with methanol and dimethyl carbonate.关键词
反胶束体系/乙酸甲酯/脂肪酶催化剂/棉籽油/生物柴油Key words
reversed micellar system/ methyl acetate/ lipase catalyst/ cottonseed oil/ biodiesel分类
化学化工引用本文复制引用
左晓旭,李芳芳,龚春霞,高剑峰..反胶束体系中脂肪酶催化乙酸甲酯与棉籽油合成生物柴油[J].石油化工,2013,42(6):604-609,6.基金项目
国家重点基础研究发展规划项目(2011-CB200900-G). （2011-CB200900-G）
