特产研究2013，Vol.35Issue(1)：41-45,5.
西洋参花药愈伤组织诱导培养条件的优化
Optimization of Anther Culture Inducing Conditions on Callus from Panax Quinquefolium L.
摘要
Abstract
Objectives of this research were to improve the induction frequency of callus and obtain optimum culture conditions in anther culture from Panax Quinquefolium L.Buds were collected as experimental materials from Ji An.Different concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) 、6-benzyladenine(6-BA) and sucrose were tested to optimize culture conditions.The results showed that the highest induction frequency 37.53％ was achieved using the medium of Murashige and Skoog basal medium(MS) + 2,4-D 2mg/L + 6-BA 0.5mg/L + Sucrose 60g/L + Agar 5.5g/L + Lactoalbumin Hdrolysate(LH)0.5g/L.2,4-D was essential factor of callus induction from American ginseng anther.The rational compounding of 2,4-D and 6-BA enhanced the induction frequency of callus.60g/L sucrose could effectively promote callus induction from American ginseng anther.关键词
西洋参/花药/愈伤组织/优化Key words
panax quinquefolium L./ anther culture/ callus/ optimum分类
农业科技引用本文复制引用
张成国,王英平,姚春林,李昌禹,雷秀娟..西洋参花药愈伤组织诱导培养条件的优化[J].特产研究,2013,35(1):41-45,5.基金项目
科技部支撑计划(2011BAI03B01) （2011BAI03B01）
