特产研究2013，Vol.35Issue(1)：46-50,5.
酵母多糖提取物HPLC指纹图谱的研究
Fingerprint Studies of the Extract of Zymosan by HPLC
摘要
Abstract
Monosaccharide composition of the extract of zymosan was determined by HPLC after preparing pre-column derivitisation as PMP derivatives.To determine the optimal testing conditions through time and temperature of derivitisation and the column and mobile phase selection.Results were analysed by the use of traditional Chinese medicine fingerprint si milarity evaluation software.The total carbohydrate of the extract of zymosan was 84.20％.Fingerprint was composed of mannose,glucose,and galactose.The similarity of the 10 batch of samples to compare with common pattern was greater than 0.9.The method was a simple,rapid,sensitive and convenient,and can be applied to the quality control of the extract of zymosan.It could also be used to determine monosaccharide composition of acidic heteropolysaccharides.关键词
酵母多糖提取物/指纹图谱/柱前衍生化/高效液相色谱Key words
the extract of zymosan/ fingerprint/ pre-column derivatization/HPLC分类
医药卫生引用本文复制引用
姜翔之,罗浩铭,周婷婷,王颖,陈英红..酵母多糖提取物HPLC指纹图谱的研究[J].特产研究,2013,35(1):46-50,5.基金项目
吉林省科技发展计划项目(20120939) （20120939）
吉林省中医药管理局中医药科技项目(2012-100) （2012-100）
